Description

o-Propargyl-Puromycin is a chemically modified puromycin analog designed for advanced biochemical research and proteomic applications. This compound is engineered to serve as a critical tool for labeling and identifying newly synthesized proteins within living cells. It is primarily utilized by researchers and scientists in the fields of chemical biology, molecular biology, and drug discovery. The product enables precise, activity-based protein profiling and is essential for studying dynamic cellular processes.

Application

• Activity-Based Protein Profiling (ABPP): A key reagent for labeling active ribosomes and nascent polypeptide chains to study protein synthesis dynamics.
• Proteomics Research: Used in mass spectrometry-based workflows for the global identification and quantification of newly synthesized proteins.
• Mechanistic Studies: Investigates the mode of action of antibiotics and the function of the ribosome in various biological systems.
• Chemical Biology Tool Development: Serves as a versatile building block for creating novel probes via click chemistry, leveraging its propargyl group.
• Drug Discovery Screening: Employed in high-throughput assays to identify compounds that modulate protein translation.
• Cellular Imaging: Facilitates the visualization of localized protein synthesis when conjugated to fluorescent reporters.

Basic Information

Product Name	o-Propargyl-Puromycin
CAS No.	1416561-90-4
Molecular Formula	C23H30N6O5
Molecular Weight	470.52 g/mol
Synonyms	6-Dimethylamino-9-[3-deoxy-3-(O-propargyltyrosylamido)-β-D-ribofuranosyl]purine; N-[(2S,3S)-3-(4-Hydroxyphenyl)-1-[(2R,3R,4R,5R)-5-(6-amino-2-dimethylaminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-1-oxobutan-2-yl]prop-2-ynamide; Propargyl-Puromycin; Puromycin, O-propargyl-; Clickable Puromycin; Alkyne-Puromycin
EINECS	Contact for details

Quality Control

Our o-Propargyl-Puromycin is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 quality management standards, and the product is suitable for use under research-grade and GLP (Good Laboratory Practice) environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, Methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.