Description

3'-o-(4,4'-Dimethoxytrityl)-2'-Deoxyinosine is a key nucleoside phosphoramidite derivative, specifically a 2'-deoxyinosine building block protected with a dimethoxytrityl (DMT) group at the 3'-hydroxyl position. This compound is essential for the solid-phase synthesis of oligonucleotides, enabling the controlled incorporation of the nucleobase inosine, which is critical for creating primers, probes, and antisense molecules with specific base-pairing properties. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in molecular biology, genetic research, and therapeutic oligonucleotide development.

Application

• Oligonucleotide Synthesis: As a phosphoramidite monomer for the automated solid-phase synthesis of DNA and RNA strands containing inosine bases.
• PCR Primer and Probe Design: For creating degenerate primers and probes where inosine acts as a universal base to reduce sequence complexity and mismatches.
• Antisense and siRNA Research: A crucial building block in the development of therapeutic oligonucleotides for gene silencing and modulation.
• Molecular Diagnostics: Used in the manufacture of diagnostic assays and kits that require specific oligonucleotide sequences for pathogen detection or genetic screening.
• Structural Biology Studies: For synthesizing oligonucleotides used in X-ray crystallography and NMR spectroscopy to study nucleic acid structure and protein-nucleic acid interactions.
• Sequencing and Genomics: Employed in the synthesis of primers and adapters for next-generation sequencing (NGS) platforms.

Basic Information

Product Name	3'-o-(4,4'-Dimethoxytrityl)-2'-Deoxyinosine
CAS No.	1401110-52-8
Molecular Formula	C31H30N4O7
Molecular Weight	570.59 g/mol
Synonyms	2'-Deoxyinosine, 3'-O-(4,4'-dimethoxytrityl)-; 3'-O-DMT-2'-deoxyinosine; DMT-dI; 9-[(2R,4S,5R)-4-([Bis(4-methoxyphenyl)(phenyl)methoxy]methyl)-5-methyloxolan-2-yl]-1,9-dihydro-6H-purin-6-one; 2'-Deoxyinosine 3'-(4,4'-dimethoxytrityl) ether; Inosine, 2'-deoxy-, 3'-(4,4'-dimethoxytrityl) ether
EINECS	Contact for details

Quality Control

Our 3'-o-(4,4'-Dimethoxytrityl)-2'-Deoxyinosine is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, suitable for sensitive oligonucleotide synthesis. Each lot is subjected to rigorous analytical testing including HPLC, NMR, and mass spectrometry to confirm identity, purity, and the absence of critical impurities. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.