Description

5'-O-(4,4'-Dimethoxytrityl)-2'-O-(2-Methoxyethyl) Adenosine is a key protected nucleoside derivative used as a critical building block in oligonucleotide synthesis. This modified adenosine monomer is essential for the production of antisense oligonucleotides and siRNA therapeutics, where it enhances nuclease resistance and pharmacokinetic properties. It is primarily utilized by pharmaceutical R&D departments, biotechnology companies, and CDMOs specializing in advanced nucleic acid chemistry.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A vital phosphoramidite precursor for automated synthesis of therapeutic oligonucleotides.
• Antisense Oligonucleotide (ASO) Development: Used to incorporate 2'-MOE (2'-O-methoxyethyl) modifications, a gold-standard chemistry for enhancing drug stability and target affinity.
• siRNA and RNAi Therapeutic Manufacturing: Serves as a protected monomer for synthesizing the sense or antisense strand of siRNA duplexes.
• Research & Development of Nucleic Acid Analogs: A fundamental reagent in academic and industrial labs for exploring novel modified nucleosides.
• GMP-Grade Active Pharmaceutical Ingredient (API) Intermediate: Employed in the cGMP production pathway for approved nucleic acid-based drugs.
• Diagnostic Probe Synthesis: Used in the manufacture of labeled probes for genetic testing and molecular diagnostics.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(2-Methoxyethyl) Adenosine
CAS No.	1384253-67-1
Molecular Formula	C35H39N5O8
Molecular Weight	657.72 g/mol
Synonyms	5'-O-DMT-2'-O-MOE-Adenosine; 2'-O-(2-Methoxyethyl)-5'-O-(4,4'-dimethoxytrityl)adenosine; 2'-MOE-5'-DMT-Adenosine; N6-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(2-methoxyethyl)adenosine (protected variant); DMT-MOE-A; Adenosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-(2-methoxyethyl)-; 2'-O-Methoxyethyl-5'-O-dimethoxytrityl Adenosine; MOE-A(DMT)
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-2'-O-(2-Methoxyethyl) Adenosine is manufactured under strict quality systems suitable for pharmaceutical development. Each batch is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry. Certificates of Analysis (COA) detailing purity, water content, and related substances are provided. We support compliance with ICH Q7 guidelines and can supply material suitable for use in GMP manufacturing upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment to prevent degradation due to its hygroscopic nature. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Individual impurity ≤ 0.5%, Total impurities ≤ 2.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.