Description

2'-o-Methyl-2-o-Ethyluridine is a chemically modified nucleoside analog, a key building block in advanced oligonucleotide synthesis. This compound is critical for researchers and manufacturers developing next-generation therapeutic and diagnostic agents, where precise chemical modification is required to enhance stability, specificity, and pharmacokinetic properties. It is primarily utilized by the pharmaceutical, biotechnology, and life science research sectors for applications in antisense oligonucleotides, siRNA, and other nucleic acid-based technologies.

Application

• Antisense Oligonucleotide (ASO) Synthesis: A crucial phosphoramidite precursor for constructing oligonucleotides with enhanced nuclease resistance and binding affinity.
• Small Interfering RNA (siRNA) Development: Used to introduce specific 2'-O-alkyl modifications to improve metabolic stability and reduce off-target effects in RNAi therapeutics.
• Diagnostic Probe Fabrication: Serves as a modified base in the synthesis of labeled probes for sensitive detection assays like FISH (Fluorescence In Situ Hybridization) and PCR.
• Medicinal Chemistry & Drug Discovery: Employed as a scaffold for exploring structure-activity relationships (SAR) in nucleoside-based drug candidates targeting viral polymerases or cellular enzymes.
• Academic & Industrial Research: A vital reagent for fundamental studies in nucleic acid chemistry, biochemistry, and molecular biology to understand modification impacts on structure and function.

Basic Information

Item	Details
Product Name	2'-o-Methyl-2-o-Ethyluridine
CAS No.	1352173-49-9
Molecular Formula	C12H18N2O6
Molecular Weight	286.28 g/mol
Synonyms	2'-O-Methyl-2-O-ethyluridine; 1-[(2R,3R,4R,5R)-3-Ethoxy-4-hydroxy-5-(methoxymethyl)oxolan-2-yl]pyrimidine-2,4-dione; 2'-O-Methyl-2-O-ethyl-uridine; 2'-O-Methyl-2-ethoxyuridine; Modified Uridine Derivative; 2'-OMe-2-OEt-uridine
EINECS	Contact for details

Quality Control

Every batch of 2'-o-Methyl-2-o-Ethyluridine is manufactured under strict quality management systems. We provide comprehensive analytical documentation to ensure the material meets the high-purity standards required for sensitive pharmaceutical and research applications. A detailed Certificate of Analysis (COA) is supplied with each shipment, reporting key parameters such as purity (by HPLC), identity (by NMR and MS), and impurity profiles.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed in a desiccated environment to prevent degradation. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.