Description

2'-o-Methyl-N2-Isobutyryl-5'-o-Dmt-Guanosine is a high-purity, chemically modified nucleoside building block essential for oligonucleotide synthesis. This compound is critical for researchers and manufacturers developing advanced therapeutic oligonucleotides, such as antisense drugs and siRNA, where precise chemical modification is required to enhance stability and efficacy. It is primarily used by pharmaceutical R&D departments, biotechnology companies, and CROs specializing in nucleic acid chemistry and drug discovery.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A key phosphoramidite precursor for the automated synthesis of modified RNA and DNA strands.
• Antisense Oligonucleotide (ASO) Development: Used to incorporate 2'-O-methyl modifications, which improve nuclease resistance and binding affinity of therapeutic ASOs.
• Small Interfering RNA (siRNA) Manufacturing: Essential for producing siRNA duplexes with enhanced pharmacokinetic profiles and reduced off-target effects.
• Research & Diagnostic Probe Synthesis: Enables the creation of highly specific, nuclease-resistant probes for molecular diagnostics and basic research.
• Pharmaceutical Intermediate: Serves as a critical raw material in the cGMP production of oligonucleotide-based active pharmaceutical ingredients (APIs).
• Medicinal Chemistry & Lead Optimization: Used to explore structure-activity relationships (SAR) in nucleic acid-based drug candidates.

Basic Information

Product Name	2'-o-Methyl-N2-Isobutyryl-5'-o-Dmt-Guanosine
CAS No.	1239669-73-8
Molecular Formula	C36H41N5O8
Molecular Weight	671.75 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-methyl-N2-isobutyrylguanosine; 2'-O-Methyl-N2-isobutyryl-5'-O-DMT-guanosine; DMT-2'-O-Me-G(ib)-CE Phosphoramidite precursor; Guanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-methyl-, 2'-(2-methylpropanoate); N2-Isobutyryl-5'-O-(4,4'-dimethoxytrityl)-2'-O-methylguanosine
EINECS	Contact for details

Quality Control

Our 2'-o-Methyl-N2-Isobutyryl-5'-o-Dmt-Guanosine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for pharmaceutical research. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry guidelines to support our customers' regulatory and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider desiccated conditions at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.