Description

3'-o-(T-Butyldimethylsilyl)-2'-o-(2-Methoxyethyl)-5-Methyluridine is a key protected nucleoside intermediate, essential for the synthesis of modified oligonucleotides and nucleotide analogs. This compound is critical for researchers and manufacturers requiring high-purity building blocks for advanced pharmaceutical development, particularly in the fields of antisense oligonucleotides and siRNA therapeutics. It is primarily utilized by biotechnology firms, pharmaceutical R&D departments, and fine chemical suppliers engaged in the production of next-generation nucleic acid-based drugs.

Application

• Pharmaceutical Intermediate: A crucial building block in the synthesis of therapeutic oligonucleotides, including antisense drugs and siRNA constructs.
• Antiviral Drug Research: Used in the development of nucleotide analogs targeting viral polymerases for potential antiviral therapies.
• Prodrug Synthesis: Serves as a protected precursor in the creation of prodrugs designed to improve the bioavailability and targeting of nucleoside-based active pharmaceutical ingredients (APIs).
• Solid-Phase Oligonucleotide Synthesis (SPOS): Employed in automated synthesizers to introduce specific, modified uridine residues into custom oligonucleotide sequences.
• Bioconjugation & Labeling: The reactive silyl and hydroxyl groups provide handles for further chemical modification, labeling, or conjugation with other molecules.
• Academic & Industrial R&D: Fundamental research tool in medicinal chemistry and molecular biology for studying nucleic acid structure, function, and drug interactions.

Basic Information

Product Name	3'-o-(T-Butyldimethylsilyl)-2'-o-(2-Methoxyethyl)-5-Methyluridine
CAS No.	1221967-92-5
Molecular Formula	C19H34N2O7Si
Molecular Weight	430.57 g/mol
Synonyms	5-Methyl-2'-O-(2-methoxyethyl)-3'-O-[(1,1-dimethylethyl)dimethylsilyl]uridine; 2'-O-MOE-5-methyl-3'-O-TBDMS-uridine; 3'-O-TBDMS-2'-O-MOE-5-methyluridine; 5-Methyluridine, 3'-O-[(1,1-dimethylethyl)dimethylsilyl]-2'-O-(2-methoxyethyl)-; TBDMS-MOE-5-Me-uridine; N1-[(2R,3R,4R,5R)-4-[(tert-Butyldimethylsilyl)oxy]-5-[hydroxy(2-methoxyethoxy)methyl]-3-methyloxolan-2-yl]-5-methylpyrimidine-2,4-dione
EINECS	Contact for details

Quality Control

Our production of 3'-o-(T-Butyldimethylsilyl)-2'-o-(2-Methoxyethyl)-5-Methyluridine adheres to stringent quality protocols to ensure batch-to-batch consistency and high purity suitable for pharmaceutical research. All materials are characterized by advanced analytical techniques including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with each shipment to support your regulatory and R&D documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider storing under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.