Description

2'-Deoxy-3',5'-Bis-o-[(1,1-Dimethylethyl)Dimethylsilyl]-5-[[[(1,1-Dimethylethyl)Dimethylsilyl]Oxy]Methyl]Cytidine is a sophisticated, multi-protected nucleoside derivative designed for advanced synthetic chemistry. This compound is critical for researchers and manufacturers requiring precise control over reactivity in the construction of oligonucleotides and other complex biomolecules. It serves as a key protected intermediate for the synthesis of modified cytidine analogs, making it essential for pharmaceutical R&D, particularly in the development of antisense oligonucleotides, siRNA, and other therapeutic nucleic acids.

Application

• Oligonucleotide Synthesis: A crucial protected building block for the solid-phase and solution-phase synthesis of DNA and RNA sequences, especially those containing modified cytidine bases.
• Antisense & siRNA Therapeutics: Used in the research and production of therapeutic oligonucleotides targeting genetic diseases, cancers, and viral infections.
• Prodrug Development: Serves as a precursor in the design of nucleoside prodrugs, where the protecting groups allow for targeted activation.
• Chemical Biology Research: Enables the site-specific incorporation of modified nucleosides into nucleic acids for structure-activity relationship (SAR) studies and mechanistic investigations.
• Diagnostic Probe Synthesis: Utilized in creating labeled nucleic acid probes for diagnostic assays and genomic research.
• Academic & Industrial R&D: A valuable reagent for synthetic organic chemists and biochemists exploring novel nucleic acid chemistry and modifications.

Basic Information

Product Name	2'-Deoxy-3',5'-Bis-o-[(1,1-Dimethylethyl)Dimethylsilyl]-5-[[[(1,1-Dimethylethyl)Dimethylsilyl]Oxy]Methyl]Cytidine
CAS No.	1210427-82-9
Molecular Formula	C31H61N3O5Si3
Molecular Weight	648.11 g/mol
Synonyms	5-[[[(1,1-Dimethylethyl)dimethylsilyl]oxy]methyl]-2'-deoxy-3',5'-bis-O-[(1,1-dimethylethyl)dimethylsilyl]cytidine; 2'-Deoxy-3',5'-bis-O-(tert-butyldimethylsilyl)-5-[(tert-butyldimethylsilyloxy)methyl]cytidine; 5-((tert-Butyldimethylsilyloxy)methyl)-2'-deoxy-3',5'-bis-O-(tert-butyldimethylsilyl)cytidine; TBDMS-protected 5-Hydroxymethyl-2'-deoxycytidine; 5-(tert-Butyldimethylsilyloxymethyl)-2'-deoxy-3',5'-di-O-(tert-butyldimethylsilyl)cytidine; 3',5'-Bis-O-TBDMS-5-(O-TBDMS-hydroxymethyl)-2'-deoxycytidine; Fully TBDMS-protected 5-Hydroxymethyl-dC; CAS 1210427-82-9
EINECS	Contact for details

Quality Control

Every batch of this high-purity synthetic intermediate is produced under strict quality management systems. We employ advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure identity, purity, and consistency. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive) and must be handled under anhydrous conditions. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.