Description

Ethynyl 2-Deoxy-5-o-Dmt-Ribosyl 3-Ce Phosphoramidite is a high-purity, specialized phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. This compound is essential for introducing modified nucleosides into synthetic DNA or RNA strands, enabling advanced research and therapeutic development. It is primarily used by pharmaceutical R&D teams, biotechnology companies, and academic research institutions focused on antisense oligonucleotides, siRNA, aptamers, and other nucleic acid-based technologies.

Application

• Oligonucleotide Synthesis: A key phosphoramidite reagent for the automated synthesis of modified DNA and RNA strands.
• Antisense Drug Development: Used to create oligonucleotides for therapeutic applications targeting gene expression.
• siRNA and miRNA Research: Essential for constructing short interfering RNA (siRNA) and microRNA (miRNA) molecules for gene silencing studies.
• Aptamer Production: Enables the synthesis of nucleic acid aptamers for diagnostic and therapeutic targeting.
• Primer and Probe Manufacturing: Used to produce labeled or modified primers and probes for molecular diagnostics and PCR applications.
• Structural Biology Studies: Facilitates the creation of oligonucleotides with specific modifications for NMR and X-ray crystallography.
• Basic Nucleic Acid Research: A fundamental tool for academic and industrial labs exploring nucleic acid chemistry and function.

Basic Information

Product Name	Ethynyl 2-Deoxy-5-o-Dmt-Ribosyl 3-Ce Phosphoramidite
CAS No.	1173797-55-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxy-5-(ethynyl)uridine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5'-O-DMT-2'-deoxy-5-ethynyluridine 3'-CE phosphoramidite; dU(Ethynyl) Phosphoramidite; 5-Ethynyl-2'-deoxyuridine CE Phosphoramidite; 5-Ethynyl-dU Phosphoramidite; Amidite for 5-ethynyl-dU; 5-EdU Phosphoramidite
EINECS	Contact for details

Quality Control

Our Ethynyl 2-Deoxy-5-o-Dmt-Ribosyl 3-Ce Phosphoramidite is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is rigorously tested using advanced analytical techniques, including HPLC and NMR, to confirm identity, purity, and stability. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere (argon or nitrogen). This material is hygroscopic (moisture-sensitive) and must be handled in a dry environment to prevent degradation. Allow the sealed vial to equilibrate to room temperature before opening to avoid condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.