Description

3'-o-Aminothymidine-5'-Triphosphate is a modified nucleotide triphosphate analog, a critical building block for advanced biochemical research and development. Its core value lies in its unique 3'-amino modification, which allows for specific enzymatic incorporation and termination, enabling precise manipulation of nucleic acid sequences. This compound is essential for researchers and manufacturers in the fields of molecular biology, next-generation sequencing (NGS), and the development of novel therapeutic oligonucleotides, such as aptamers and antisense agents.

Application

• Next-Generation Sequencing (NGS): Used as a reversible terminator in sequencing-by-synthesis platforms to enable accurate base calling.
• Oligonucleotide Synthesis: Serves as a modified monomer for the enzymatic synthesis of specialized DNA strands with tailored functionalities.
• Aptamer Development: Incorporated into SELEX (Systematic Evolution of Ligands by Exponential Enrichment) libraries to create high-affinity nucleic acid ligands for diagnostic and therapeutic targets.
• Antisense & RNAi Research: Utilized in the study and development of oligonucleotide-based drugs that modulate gene expression.
• PCR & Enzymatic Labeling: Acts as a substrate for DNA polymerases in specialized PCR applications and enzymatic labeling techniques.
• Structural Biology Studies: Employed to probe enzyme mechanisms and study the structure-function relationships of nucleic acid-processing enzymes.

Basic Information

Product Name	3'-o-Aminothymidine-5'-Triphosphate
CAS No.	1159990-37-0
Molecular Formula	C10H17N3O13P3
Molecular Weight	504.17 g/mol
Synonyms	3'-Amino-2',3'-dideoxythymidine-5'-triphosphate; 3'-NH2-dTTP; 3'-Amino-ddTTP; Aminothymidine triphosphate; 3'-Amino-2',3'-dideoxy-5'-TTP; 2',3'-Dideoxy-3'-aminothymidine-5'-triphosphate; 3’-Amino-2’,3’-dideoxythymidine 5’-triphosphate
EINECS	Contact for details

Quality Control

Our 3'-o-Aminothymidine-5'-Triphosphate is manufactured under strict quality management systems to ensure the high purity and consistency required for sensitive research applications. Each batch is subjected to rigorous analytical testing, including HPLC for purity assessment and identity confirmation via mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, impurity profile, and analytical methods is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 5.0%
Triphosphate Content (³¹P-NMR)	≥ 85%
Biological Activity (Enzymatic Incorporation)	Suitable for use

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.