Description

Azido-PEG3-CH2CO2-NHS is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and an N-hydroxysuccinimide (NHS) ester. This compound is engineered for efficient and selective bioconjugation, enabling the stable linkage of biomolecules through click chemistry and amine-reactive coupling. It is an essential reagent for researchers and manufacturers in biopharmaceutical development, proteomics, and advanced material science, where precise and stable molecular assembly is critical.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Development: Used to link cytotoxic drugs, fluorophores, or other payloads to antibodies, proteins, or peptides via the azide-alkyne cycloaddition (click chemistry) and NHS-ester amine coupling.
• PEGylation of Biomolecules: Serves as a spacer to introduce PEG chains onto proteins, peptides, or nanoparticles, improving solubility, stability, and pharmacokinetic profiles.
• Surface Functionalization: Employed to modify surfaces (e.g., biosensors, microarrays, gold nanoparticles) with azide or amine-reactive groups for subsequent immobilization of biomolecules.
• Polymer and Hydrogel Synthesis: Acts as a crosslinking agent in the creation of functionalized polymers and biocompatible hydrogels for drug delivery and tissue engineering.
• Chemical Biology and Proteomics: A key tool for labeling, pull-down assays, and activity-based protein profiling to study protein function and interactions.
• Diagnostic Reagent Formulation: Utilized in the development of diagnostic kits and assays that require stable, site-specific labeling of detection molecules.

Basic Information

Product Name	Azido-PEG3-CH2CO2-NHS
CAS No.	1092654-47-1
Molecular Formula	C16H24N4O8
Molecular Weight	400.39 g/mol
Synonyms	NHS-PEG3-Azide; Azido-PEG3-NHS ester; 3-Azidopropyl-PEG3-NHS ester; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]propanoate; 11-Azido-3,6,9-trioxaundecanoic acid N-hydroxysuccinimide ester; N-(3-Azidopropyl)-3,6,9-trioxaundecanedioic acid imide; PEG3-Azide-NHS; Azido-tri(ethylene glycol)-NHS ester
EINECS	Contact for details

Quality Control

Our Azido-PEG3-CH2CO2-NHS is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and functional group analysis, to ensure high batch-to-batch consistency and performance. Certificates of Analysis (COA) with detailed specifications are provided to guarantee compliance with your research and production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and the NHS ester is susceptible to hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or oil
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Functional Group Assay (Azide)	≥90%
Functional Group Assay (NHS Ester)	≥90%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.