Description

Thymidine 5'-(Tetrahydrogen Triphosphate) 3'-(Dihydrogen Phosphate) is a high-purity nucleotide derivative essential for advanced biochemical research and pharmaceutical development. This compound serves as a critical building block and substrate in enzymatic reactions, particularly those involving DNA polymerases and kinases. It is primarily utilized by research institutions, diagnostic kit manufacturers, and biotechnology companies engaged in molecular biology, enzymology, and next-generation sequencing (NGS) applications.

Application

• Enzymatic Synthesis: As a specialized substrate for DNA polymerases in the synthesis of oligonucleotides and modified DNA strands.
• Biochemical Research: Used in studies of enzyme kinetics, mechanism, and inhibition for nucleotide-processing enzymes.
• Diagnostic Assay Development: A key component in the formulation of reagents for molecular diagnostics and PCR-based tests.
• Next-Generation Sequencing (NGS): Employed in sequencing-by-synthesis platforms as a terminator or reversible terminator nucleotide.
• Pharmaceutical Intermediates: Serves as a precursor in the synthesis of novel antiviral or anticancer nucleoside analogs.
• Labeling & Detection: Can be modified with fluorescent or radioactive tags for use as probes in hybridization and detection assays.

Basic Information

Product Name	Thymidine 5'-(Tetrahydrogen Triphosphate) 3'-(Dihydrogen Phosphate)
CAS No.	1015762-49-8
Molecular Formula	C10H18N2O17P4
Molecular Weight	604.14 g/mol
Synonyms	Thymidine 5'-triphosphate 3'-monophosphate; dTTP-3'-monophosphate; 3'-dTMP-5'-triphosphate; 2'-Deoxythymidine-5'-triphosphate-3'-monophosphate; Thymidine 5'-(trihydrogen triphosphate) 3'-(hydrogen phosphate); TTP-3'-P; dTTP-3'-P; 5'-O-({[({[(2R,3S,5R)-3-Hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)oxolan-2-yl]methoxy}(hydroxy)phosphoryl)oxy](hydroxy)phosphoryl}oxy)phosphono-2'-deoxythymidine
EINECS	Contact for details

Quality Control

Our Thymidine 5'-(Tetrahydrogen Triphosphate) 3'-(Dihydrogen Phosphate) is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the exacting standards required for sensitive research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95%
Assay (UV Spectrophotometry)	≥ 90%
Water Content (Karl Fischer)	≤ 5.0%
Solubility	Soluble in water, slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.