Description

N4-Acetyl-5'-(Dimethoxytrityl)-2'-o-Methylcytidine is a key protected nucleoside derivative used as a critical building block in oligonucleotide synthesis. This compound matters for its role in the production of chemically modified RNA strands, which are essential for advanced therapeutic and diagnostic applications. It is primarily needed by pharmaceutical R&D teams, biotechnology companies, and CDMOs specializing in the development of antisense oligonucleotides, siRNA, and other nucleic acid-based therapeutics.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A vital phosphoramidite precursor for the automated synthesis of RNA sequences containing 2'-O-methyl modifications.
• Antisense Oligonucleotide (ASO) Development: Used in the research and manufacturing of ASOs designed for targeted gene silencing.
• siRNA and miRNA Therapeutics: Serves as a protected monomer for constructing siRNA duplexes with enhanced nuclease resistance and pharmacokinetic profiles.
• Diagnostic Probe Synthesis: Employed in creating labeled nucleic acid probes for research, clinical diagnostics, and in-situ hybridization.
• Medicinal Chemistry & Drug Discovery: Acts as a scaffold for exploring novel nucleoside analogs with potential antiviral or anticancer activity.
• GMP-Grade API Manufacturing: A starting material or intermediate in the cGMP production of oligonucleotide active pharmaceutical ingredients (APIs).

Basic Information

Product Name	N4-Acetyl-5'-(Dimethoxytrityl)-2'-o-Methylcytidine
CAS No.	199593-08-3
Molecular Formula	C39H41N3O8
Molecular Weight	679.76 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-acetyl-2'-O-methylcytidine; 2'-O-Methyl-N4-acetyl-5'-O-DMT-cytidine; DMT-2'-O-Me-N4-Ac-C; 2'-O-Methyl-N4-acetylcytidine 5'-O-(4,4'-dimethoxytrityl) ether; N4-Ac-5'-DMT-2'-OMe-Cytidine; 1-[(2R,3R,4R,5R)-5-[[Bis(4-methoxyphenyl)-phenylmethoxy]methyl]-3,4-dihydroxy-4-methoxyoxolan-2-yl]-4-acetamidopyrimidin-2-one
EINECS	Contact for details

Quality Control

Our N4-Acetyl-5'-(Dimethoxytrityl)-2'-o-Methylcytidine is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability, consistency, and compliance with your research or cGMP needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, desiccants are recommended.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.