Description

5'-o-(4,4'-Dimethoxytrityl)-2'-O,4'-C-Methylene Uridine is a sophisticated, chemically modified nucleoside derivative, serving as a critical building block in oligonucleotide synthesis. This compound is essential for the production of antisense oligonucleotides, siRNA, and other therapeutic nucleic acids, where it provides enhanced nuclease resistance and binding affinity. It is a key raw material for pharmaceutical R&D and manufacturing, particularly for companies developing next-generation genetic medicines and molecular diagnostics.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A vital phosphoramidite precursor for the automated synthesis of antisense oligonucleotides (ASOs) and small interfering RNA (siRNA).
• Therapeutic Nucleic Acid Development: Used in the research and production of locked nucleic acid (LNA) or bridged nucleic acid (BNA) analogs for enhanced stability and efficacy in drug candidates.
• Diagnostic Probe Manufacturing: Incorporated into high-specificity probes for PCR, FISH, and microarray technologies.
• Academic & Industrial Research: A fundamental reagent for studying RNA structure, function, and for developing novel nucleic acid-based tools.
• Contract Development and Manufacturing (CDMO): Supplied to CDMOs specializing in GMP-grade oligonucleotide production for clinical and commercial applications.

Basic Information

Product Name	5'-o-(4,4'-Dimethoxytrityl)-2'-O,4'-C-Methylene Uridine
CAS No.	195705-32-9
Molecular Formula	C32H32N2O8
Molecular Weight	572.61 g/mol
Synonyms	5'-O-DMT-2'-O,4'-C-Methyleneuridine; 2'-O,4'-C-Methylene-5'-O-(4,4'-dimethoxytrityl)uridine; 5'-O-(4,4'-Dimethoxytrityl)-2'-O,4'-C-methyleneuridine; DMT-LNA-Uridine; LNA-Uridine DMT Protected; BNA-Uridine (DMT); 5'-DMT-2',4'-BNA-U; 5'-O-Dimethoxytrityl-2'-O,4'-C-methyleneuridine
EINECS	Contact for details

Quality Control

Our 5'-o-(4,4'-Dimethoxytrityl)-2'-O,4'-C-Methylene Uridine is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive pharmaceutical applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing identity, purity (typically ≥98% by HPLC), and impurity profiles. We adhere to cGMP guidelines where applicable, and our quality management system is designed to meet the stringent requirements of global regulatory frameworks.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under a dry inert atmosphere (e.g., nitrogen or argon) when opening to prevent degradation. For long-term storage, desiccants are recommended.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Purity (HPLC, Total)	≥ 99.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH Q3C guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.