Description

5'-O-DMT-N4-Ethyl-2'-Deoxycytidine is a high-purity, protected nucleoside phosphoramidite precursor, a critical building block in automated oligonucleotide synthesis. This compound is essential for the precise and efficient solid-phase synthesis of DNA and RNA sequences, enabling the incorporation of modified bases. It is primarily used by research institutions, diagnostic developers, and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNAs, aptamers, and other advanced therapeutic modalities.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the solid-phase synthesis of custom DNA and RNA strands.
• Therapeutic Nucleic Acid Development: For the research and production of antisense oligonucleotides, siRNA, and aptamer-based therapeutics.
• Diagnostic Probe Manufacturing: Used in creating labeled probes for PCR, FISH, and microarray technologies.
• Structural Studies: Incorporation into oligonucleotides for NMR and X-ray crystallography to study nucleic acid structure and protein interactions.
• Synthetic Biology: Enables the construction of novel genetic circuits and engineered biomolecules with modified functionalities.
• Basic Biochemical Research: A vital reagent for studying DNA replication, repair mechanisms, and enzyme kinetics.

Basic Information

Product Name	5'-O-DMT-N4-Ethyl-2'-Deoxycytidine
CAS No.	195535-75-2
Molecular Formula	C34H39N3O6
Molecular Weight	585.70 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-ethyl-2'-deoxycytidine; N4-Ethyl-5'-O-DMT-2'-deoxycytidine; DMT-dC(Et) Phosphoramidite precursor; 2'-Deoxy-N4-ethyl-5'-O-(4,4'-dimethoxytrityl)cytidine; N4-Ethyl-2'-deoxy-5'-O-(4,4'-dimethoxytrityl)cytidine; 5'-O-DMT-N4-Et-dC; N4-Ethyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine
EINECS	Contact for details

Quality Control

Our 5'-O-DMT-N4-Ethyl-2'-Deoxycytidine is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive oligonucleotide synthesis. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and critical impurity profiles. We adhere to cGMP principles where applicable, and our quality control protocols are designed to meet the stringent requirements of pharmaceutical and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic and light-sensitive; allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, DCM, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.