Description

Uridine, 5'-Azido-5'-Deoxy-2'-o-Methyl- CAS NO 194034-68-9 is a chemically modified nucleoside analog, specifically engineered with an azido group at the 5' position and a methyl group at the 2' oxygen. This compound is a critical building block and click chemistry reagent for the synthesis and modification of oligonucleotides, enabling advanced research in nucleic acid chemistry and drug discovery. It is primarily utilized by pharmaceutical R&D teams, academic researchers, and biotechnology companies focused on developing novel therapeutics, diagnostic probes, and functional biomaterials.

Application

• Oligonucleotide Synthesis: Key intermediate for the solid-phase synthesis of modified RNA and DNA strands, particularly for introducing functional handles via click chemistry.
• Antiviral & Anticancer Drug Development: Serves as a precursor in the research and development of nucleoside-based prodrugs targeting viral polymerases and cellular replication mechanisms.
• Bioconjugation & Labeling: The 5'-azido moiety allows for efficient, site-specific conjugation with alkyne-bearing fluorophores, affinity tags, or delivery vehicles using copper-catalyzed azide-alkyne cycloaddition (CuAAC).
• Structural Biology Studies: Used to incorporate stable modifications into RNA for NMR and X-ray crystallography studies to understand structure-function relationships.
• siRNA & miRNA Therapeutics: Employed in the design of stabilized siRNA and miRNA mimics with enhanced nuclease resistance and pharmacokinetic properties.
• Chemical Biology Probes: Facilitates the creation of activity-based probes to study RNA-protein interactions and metabolic pathways within cells.

Basic Information

Product Name	Uridine, 5'-Azido-5'-Deoxy-2'-o-Methyl- CAS NO 194034-68-9
CAS No.	194034-68-9
Molecular Formula	C11H15N5O5
Molecular Weight	297.27 g/mol
Synonyms	5'-Azido-5'-deoxy-2'-O-methyluridine; 2'-O-Methyl-5'-azido-5'-deoxyuridine; 5'-Azido-2'-O-methyl-5'-deoxyuridine; 1-((2R,3R,4R,5R)-5-Azido-3,4-dihydroxy-4-methoxytetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione; 2'-O-Methyl-5'-azidouridine; Azido-modified uridine; Click chemistry uridine reagent
EINECS	Contact for details

Quality Control

Our Uridine, 5'-Azido-5'-Deoxy-2'-o-Methyl- is manufactured under strict quality systems suitable for research and development applications. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and structural confirmation via NMR and Mass Spectrometry. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and impurity profiles to ensure traceability and compliance with your research protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.