Description

5'-O-(4,4'-Dimethoxytrityl)-2,3'-Anhydrothymidine is a sophisticated nucleoside derivative and a crucial protected intermediate in oligonucleotide synthesis. This compound matters because it enables the precise and efficient construction of DNA sequences, particularly for antisense oligonucleotides and other therapeutic nucleic acids. Pharmaceutical researchers, biotechnology firms, and fine chemical manufacturers specializing in nucleic acid-based drug development and molecular biology reagents are the primary users of this high-value building block.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the solid-phase synthesis of therapeutic and diagnostic DNA strands.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) targeting specific mRNA sequences.
• siRNA and miRNA Synthesis: Serves as a protected monomer for constructing short interfering RNA (siRNA) and microRNA (miRNA) molecules.
• Molecular Biology Probes: Manufacture of labeled probes and primers for PCR, sequencing, and hybridization assays.
• Pharmaceutical Intermediates: A critical building block in the multi-step synthesis of novel nucleoside-based pharmaceuticals.
• Research & Development: Used in academic and industrial R&D for studying nucleic acid chemistry and developing new synthetic methodologies.

Basic Information

Item	Detail
Product Name	5'-O-(4,4'-Dimethoxytrityl)-2,3'-Anhydrothymidine
CAS No.	191474-13-2
Molecular Formula	C33H34N2O7
Molecular Weight	570.64 g/mol
Synonyms	5'-O-DMT-2,3'-Anhydrothymidine; 2,3'-Anhydro-5'-O-(4,4'-dimethoxytrityl)thymidine; DMT-Anhydrothymidine; 5'-O-(Bis(4-methoxyphenyl)phenylmethoxy)-2,3'-anhydrothymidine; 2,3'-O-Anhydro-5'-O-(4,4'-dimethoxytrityl)thymidine; Thymidine, 2,3'-anhydro-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-; Anhydro DMT-Thymidine
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-2,3'-Anhydrothymidine is produced under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for pharmaceutical applications. All products undergo rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under a dry inert atmosphere (e.g., nitrogen or argon) when opening the primary container to prevent degradation. For long-term storage, desiccants are recommended.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.