Description

1-(β-D-2-Deoxyribofuranosyl)-5-Nitroindole is a specialized nucleoside analog, a modified deoxyribonucleoside where a 5-nitroindole base replaces the canonical purine or pyrimidine. This compound matters as a critical building block and research tool in nucleic acid chemistry, enabling the study of non-canonical base pairing, mutagenesis, and the development of functional oligonucleotides. It is needed by pharmaceutical R&D teams, diagnostic developers, and academic researchers focused on antisense technology, probe design, and expanding the genetic alphabet for synthetic biology applications.

Application

• Oligonucleotide Synthesis: Serves as a phosphoramidite precursor for the solid-phase synthesis of modified DNA strands containing non-natural bases.
• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies with polymerases, nucleoside kinases, and other nucleic acid-metabolizing enzymes.
• Antisense & Diagnostic Probe Development: Incorporated into oligonucleotide probes to modulate hybridization properties, enhance specificity, or introduce reporter functionalities.
• Mutagenesis Studies: Acts as a universal or ambiguous base analog to investigate DNA replication fidelity and mutation mechanisms.
• Medicinal Chemistry: Functions as a key intermediate in the development of nucleoside-based antiviral or anticancer prodrug candidates.
• Synthetic Biology: A component in creating artificial genetic systems and xenonucleic acids (XNAs) with novel properties.

Basic Information

Product Name	1-(β-D-2-Deoxyribofuranosyl)-5-Nitroindole
CAS No.	191421-10-0
Molecular Formula	C₁₃H₁₃N₃O₆
Molecular Weight	307.26 g/mol
Synonyms	5-Nitroindole-2'-deoxyriboside; 2'-Deoxy-5-nitroindole riboside; 1-(2-Deoxy-β-D-ribofuranosyl)-5-nitro-1H-indole; 5-Nitroindole deoxyribonucleoside; d5NI; 5-Nitroindole-2'-deoxyribonucleoside; 1-(2-Deoxy-β-D-erythro-pentofuranosyl)-5-nitro-1H-indole
EINECS	Contact for details

Quality Control

Our 1-(β-D-2-Deoxyribofuranosyl)-5-Nitroindole is produced under a quality management system and undergoes rigorous analytical testing to ensure batch-to-batch consistency and high purity suitable for research and development. Standard quality control includes identity confirmation by spectroscopic methods and purity assessment by HPLC. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each batch to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccant or inert atmosphere conditions.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.