Description

2'-Deoxy-5'-o-Dmt-N6-Isobutyryladenosine is a high-purity, protected nucleoside phosphoramidite precursor, a critical building block in oligonucleotide synthesis. Its value lies in enabling the precise and efficient assembly of DNA sequences for research and therapeutic applications. This compound is essential for biotechnology and pharmaceutical companies engaged in the development of antisense oligonucleotides, primers, probes, and other synthetic genetic materials. Consistent quality and reliable supply are paramount for ensuring successful synthesis outcomes and downstream applications.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA strands.
• Antisense Oligonucleotide (ASO) Development: For the research and production of therapeutic oligonucleotides targeting specific RNA sequences.
• Primer and Probe Manufacturing: Used in the synthesis of high-fidelity primers for PCR and labeled probes for diagnostic assays.
• siRNA and miRNA Synthesis: A fundamental component in creating short interfering RNA (siRNA) and microRNA (miRNA) constructs for gene silencing studies.
• Research Scale Synthesis: For academic and industrial R&D requiring custom DNA sequences.
• GMP-Grade Therapeutic Production: Supplied under controlled conditions for use in Good Manufacturing Practice (GMP) processes for clinical-stage and commercial therapeutics.

Basic Information

Product Name	2'-Deoxy-5'-o-Dmt-N6-Isobutyryladenosine
CAS No.	190834-10-7
Molecular Formula	C38H43N5O7
Molecular Weight	681.78 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N6-isobutyryl-2'-deoxyadenosine; dA(Ibu) Phosphoramidite (protected precursor); N6-Isobutyryl-5'-O-DMT-2'-deoxyadenosine; DMTr-dA(Ib)-CE Phosphoramidite (after activation); 2'-Deoxyadenosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N-[ (2-methyl-1-oxopropyl)]-; Protected dA Monomer; Adenosine, 2'-deoxy-, N6-(2-methyl-1-oxopropyl)-, 5'-[bis(4-methoxyphenyl)phenylmethyl] ether
EINECS	Contact for details

Quality Control

Every batch of 2'-Deoxy-5'-o-Dmt-N6-Isobutyryladenosine is manufactured and tested to meet stringent quality standards suitable for sensitive oligonucleotide synthesis. Our quality assurance protocol includes comprehensive analytical testing via HPLC, NMR, and mass spectrometry to ensure identity, purity, and absence of critical impurities. A Certificate of Analysis (COA) detailing all test results is provided with each shipment. We support compliance requirements for research and cGMP applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive); allow the sealed container to equilibrate to room temperature before opening to prevent condensation. For optimal performance, store under an inert atmosphere such as argon or nitrogen after initial opening.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.