Description

5'-Azido-5'-Deoxy-2'-o-Methyl-5-Methyluridine is a chemically modified nucleoside analog, a key building block in oligonucleotide synthesis and medicinal chemistry research. Its unique structure, featuring an azido group at the 5'-position and a 2'-O-methyl modification, makes it a critical intermediate for the development of therapeutic nucleic acids, including antisense oligonucleotides and siRNA. This compound is essential for researchers and manufacturers in the pharmaceutical and biotechnology sectors focused on next-generation genetic medicines and diagnostic probes.

Application

• Oligonucleotide Synthesis: A vital phosphoramidite precursor for the solid-phase synthesis of modified RNA and DNA strands, enabling the incorporation of specific functionalities.
• Antisense & siRNA Therapeutics: Used to create metabolically stable, nuclease-resistant oligonucleotides for gene silencing and therapeutic applications.
• Click Chemistry Conjugation: The 5'-azido group allows for efficient, site-specific bioconjugation via copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-labeled probes, dyes, or delivery vehicles.
• Medicinal Chemistry & Drug Discovery: Serves as a scaffold for developing novel nucleoside-based antiviral and anticancer agents.
• Diagnostic Probe Development: Utilized in the creation of labeled nucleic acid probes for imaging, detection, and diagnostic assays.
• Biochemical Research: A tool compound for studying RNA structure, function, and metabolism, as well as enzyme-substrate interactions.

Basic Information

Product Name	5'-Azido-5'-Deoxy-2'-o-Methyl-5-Methyluridine
CAS No.	187733-73-9
Molecular Formula	C11H15N5O5
Molecular Weight	297.27 g/mol
Synonyms	5'-Azido-5'-deoxy-2'-O-methyl-5-methyluridine; 2'-O-Methyl-5-methyluridine 5'-azido-5'-deoxy; 5-Methyl-2'-O-methyluridine 5'-azido-5'-deoxy; 5'-N3-5'-d-2'-OMe-5-MeU; Azido-modified nucleoside; 1-[(2R,3R,4R,5R)-5-Azido-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methyl-1,2,3,4-tetrahydropyrimidine-2,4-dione
EINECS	Contact for details

Quality Control

Our 5'-Azido-5'-Deoxy-2'-o-Methyl-5-Methyluridine is produced under stringent quality management systems to ensure batch-to-batch consistency and high purity for research and cGMP-grade applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for advanced pharmaceutical intermediates, supporting our commitment to supply reliability and technical support.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). For long-term storage under inert atmosphere, consider storing at -20°C. Keep the container tightly sealed in a dry environment to protect this moisture-sensitive compound.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.