Description

5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxy-2'-Fluoro-5-Methyluridine-3'-O-[(2-Cyanoethyl)-(N,N-Diisopropyl)]-Phosphoramidite is a high-purity, modified nucleoside phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is specifically engineered to introduce a 2'-fluoro-5-methyluridine modification, which significantly enhances the nuclease resistance, thermal stability, and binding affinity of the resulting synthetic RNA or DNA strands. It is a critical building block for research and development in therapeutic oligonucleotides, including antisense drugs, siRNA, and aptamers, where precise chemical modification dictates biological activity and pharmacokinetic properties.

Application

• Therapeutic Oligonucleotide Synthesis: Primary building block for manufacturing antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and microRNA mimics for pharmaceutical applications.
• Research & Diagnostic Probes: Used to synthesize fluoro-modified probes for fluorescence in situ hybridization (FISH), Northern/Southern blotting, and real-time PCR, offering enhanced specificity and stability.
• Aptamer Development: Key reagent in creating nuclease-resistant aptamers for targeted drug delivery, biosensing, and diagnostic assays.
• Structural Biology Studies: Enables the production of modified RNA strands for X-ray crystallography and NMR studies to understand RNA-protein interactions and folding.
• Next-Generation Sequencing (NGS): Used in the synthesis of specialized primers and adapters that require improved thermal and enzymatic stability.
• Bioconjugation & Labeling: Serves as a precursor for synthesizing oligonucleotides with specific reactive handles for conjugation with dyes, peptides, or other molecules.

Basic Information

Item	Information
Product Name	5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxy-2'-Fluoro-5-Methyluridine-3'-O-[(2-Cyanoethyl)-(N,N-Diisopropyl)]-Phosphoramidite
CAS No.	182700-06-7
Molecular Formula	C41H48FN4O8P
Molecular Weight	774.82 g/mol
Synonyms	2'-Fluoro-5-methyluridine Phosphoramidite; 2'-F-5-Me-U Phosphoramidite; 5'-DMT-2'-F-5-methyluridine-3'-CE Phosphoramidite; 2'-Deoxy-2'-fluoro-5-methyluridine 3'-(2-cyanoethyl N,N-diisopropyl)phosphoramidite 5'-O-(4,4'-dimethoxytrityl) ether; 2'-F-5-MeU Phosphoramidite; 5-Methyl-2'-fluorouridine Phosphoramidite; 2'-Fluoro-2'-deoxy-5-methyluridine Phosphoramidite
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxy-2'-Fluoro-5-Methyluridine-3'-O-[(2-Cyanoethyl)-(N,N-Diisopropyl)]-Phosphoramidite is manufactured under strict quality systems suitable for pharmaceutical research. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and MS, to ensure compliance with stringent industry standards. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee batch-to-batch consistency and support your regulatory filings.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.