Description

5'-O-(4,4'-Dimethoxytrityl)-2'-O-(2-Methoxyethyl)-5-Methylcytidine is a key protected nucleoside derivative, specifically a 5-methylcytidine analog modified with orthogonal protecting groups. This compound is essential for the solid-phase synthesis of oligonucleotides, particularly for research and development of antisense and siRNA therapeutics. It is primarily used by pharmaceutical R&D teams, biotechnology companies, and academic research institutions focused on nucleic acid chemistry and drug discovery.

Application

• Oligonucleotide Synthesis: A critical phosphoramidite precursor for the automated solid-phase synthesis of modified RNA strands.
• Antisense Therapeutic Development: Used in the research and production of antisense oligonucleotides (ASOs) targeting specific genes.
• siRNA and RNAi Research: A vital building block for synthesizing small interfering RNA (siRNA) with enhanced nuclease resistance and pharmacokinetic properties.
• Pharmaceutical Intermediates: Serves as a high-value intermediate in the manufacturing pathway for novel nucleic acid-based drugs.
• Bioconjugation and Probe Development: Used to create labeled nucleotides for diagnostic assays and molecular biology research tools.
• Academic and Industrial R&D: Fundamental material for studying structure-activity relationships (SAR) in modified nucleosides and nucleotides.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(2-Methoxyethyl)-5-Methylcytidine
CAS No.	182496-00-0
Molecular Formula	C35H41N3O8
Molecular Weight	631.72 g/mol
Synonyms	5'-O-DMT-2'-O-MOE-5-Methylcytidine; 2'-O-(2-Methoxyethyl)-5-methylcytidine 5'-O-(4,4'-dimethoxytrityl) ether; 5-Methyl-2'-O-(2-methoxyethyl)cytidine DMT-protected; DMT-2'-MOE-5-Me-C; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(2-methoxyethyl)-5-methylcytidine (protected variant); 5-Me-C(MOE) DMT; RNA Phosphoramidite Synthon
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-2'-O-(2-Methoxyethyl)-5-Methylcytidine is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP guidelines where applicable to support our customers' regulatory and research needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. Allow the sealed container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Single Maximum Impurity	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.