Description

2'-o-(tert-Butyldimethylsilyl)-n-Isobutyrylguanosine is a key protected nucleoside derivative, specifically engineered for oligonucleotide synthesis. This compound is critical for researchers and manufacturers requiring precise control over guanosine reactivity during the assembly of DNA and RNA sequences. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic sectors for applications in antisense therapy, siRNA development, and molecular diagnostics.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): Serves as a crucial phosphoramidite building block for the automated synthesis of DNA and RNA strands.
• Antisense Oligonucleotide (ASO) Production: Used in the manufacture of therapeutic oligonucleotides designed to target specific mRNA sequences.
• Small Interfering RNA (siRNA) Development: A fundamental raw material for constructing siRNA duplexes used in gene silencing research and therapeutics.
• Diagnostic Probe Synthesis: Employed in creating labeled probes for techniques like FISH (Fluorescence In Situ Hybridization) and PCR.
• Research & Development: Essential for academic and industrial R&D focused on nucleic acid chemistry, modified nucleotides, and novel therapeutic modalities.
• Scale-Up cGMP Manufacturing: Supplied under controlled conditions for use in the production of active pharmaceutical ingredients (APIs) for clinical trials and commercial drugs.

Basic Information

Product Name	2'-o-(tert-Butyldimethylsilyl)-n-Isobutyrylguanosine
CAS No.	182007-86-9
Molecular Formula	C21H35N5O6Si
Molecular Weight	481.63 g/mol
Synonyms	2'-O-TBDMS-N2-Isobutyryl-guanosine; N2-Isobutyryl-2'-O-(tert-butyldimethylsilyl)guanosine; Guanosine, 2'-O-[(1,1-dimethylethyl)dimethylsilyl]-N-(2-methyl-1-oxopropyl)-; 2'-O-TBDMS-G(ib); N-Isobutyryl-2'-O-tert-butyldimethylsilylguanosine; Protected Guanosine Derivative; TBDMS-G(ib) Phosphoramidite Precursor
EINECS	Contact for details

Quality Control

Every batch of 2'-o-(tert-Butyldimethylsilyl)-n-Isobutyrylguanosine is manufactured and tested under strict quality management systems. Our products undergo rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure identity, purity, and consistency. Certificates of Analysis (COA) with full traceability are provided and can be tailored to meet specific customer or regulatory requirements, including those for cGMP production.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.