Description

2'-Deoxy-5'-o-Dmt-N2-Isobutyrylguanosine 3'-H Phosphonate Triethylammonium Salt is a high-purity, phosphoramidite-protected nucleoside building block essential for automated oligonucleotide synthesis. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of DNA strands. It is primarily required by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic research institutions engaged in antisense therapy, PCR primer development, and genetic analysis.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated, stepwise assembly of DNA sequences on solid support.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides (ASOs), siRNA, and aptamers for drug discovery and development.
• Diagnostic Probe & Primer Synthesis: Fabrication of labeled probes and primers for PCR, qPCR, sequencing, and microarray applications.
• Research-Grade Oligo Production: Synthesis of custom DNA fragments for academic and industrial research in molecular biology and genetics.
• Synthesis of Modified Oligonucleotides: Enabling the incorporation of site-specific modifications for stability and functional studies.

Basic Information

Product Name	2'-Deoxy-5'-o-Dmt-N2-Isobutyrylguanosine 3'-H Phosphonate Triethylammonium Salt
CAS No.	181269-06-7
Molecular Formula	C41H53N6O9P · C6H15N
Molecular Weight	~ 887.0 g/mol (as free acid)
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxy-N2-isobutyrylguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dG-Ibu Phosphoramidite; Guanosine, 2'-deoxy-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N2-(2-methyl-1-oxopropyl)-, 3'-[2-cyanoethyl (diisopropylamino)phosphoramidite]; Iso-dG-CE Phosphoramidite; N2-Isobutyryl-5'-O-DMT-2'-deoxyguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dG(ib) Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of our nucleoside phosphoramidites is produced under strict quality management systems. We guarantee high purity and consistency, supported by comprehensive analytical testing including HPLC, ¹H/³¹P NMR, and MS. A Certificate of Analysis (COA) detailing purity, moisture content, and performance in coupling tests is provided with each shipment to ensure it meets the stringent requirements for oligonucleotide synthesis.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Due to its hygroscopic and moisture-sensitive nature, allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (³¹P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile
Coupling Efficiency	≥ 99.0% (by trityl assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.