Description

5'-o-(4,4-Dimethoxytrityl)-n-[[4-(tert-Butyl)Phenoxy]Acetyl]-2'-o-Methylcytidine-3'-(2-Cyanoethyl-N,n-Diisopropyl)Phosphoramidite is a high-purity, chemically modified nucleoside phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound matters because it enables the precise and efficient incorporation of a 2'-O-methylcytidine modification, a critical feature for enhancing the stability, specificity, and therapeutic efficacy of synthetic RNA and antisense oligonucleotides. It is a vital raw material for pharmaceutical R&D teams, diagnostic developers, and CROs focused on creating advanced nucleic acid-based therapeutics, including siRNA, antisense drugs, and aptamers.

Application

• Therapeutic Oligonucleotide Synthesis: Key building block for the solid-phase synthesis of antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) drug candidates.
• Research & Diagnostic Probes: Manufacture of modified DNA/RNA probes with enhanced nuclease resistance for sensitive diagnostic assays and basic research applications.
• Aptamer Development: Used in the synthesis of chemically modified aptamers for therapeutic targeting and biosensing.
• Splice-Switching Oligonucleotides (SSOs): Critical for producing SSOs used in treatments for genetic disorders by modulating RNA splicing.
• Good Manufacturing Practice (GMP) Production: Suitable for use in GMP-grade manufacturing processes for clinical and commercial-stage oligonucleotide APIs.
• Synthesis of Chimeric Oligomers: Enables the creation of oligonucleotides with mixed backbone chemistries (e.g., DNA/2'-O-Me RNA chimeras) to optimize pharmacokinetic properties.

Basic Information

Item	Information
Product Name	5'-o-(4,4-Dimethoxytrityl)-n-[[4-(tert-Butyl)Phenoxy]Acetyl]-2'-o-Methylcytidine-3'-(2-Cyanoethyl-N,n-Diisopropyl)Phosphoramidite
CAS No.	179486-26-1
Molecular Formula	C55H67N4O9P
Molecular Weight	959.12 g/mol
Synonyms	2'-O-Methyl-5'-O-(4,4'-dimethoxytrityl)-N4-[4-(tert-butyl)phenoxyacetyl]cytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-Me-C(PAC) Phosphoramidite; N4-(4-tert-Butylphenoxyacetyl)-2'-O-methylcytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-Methyl-C(PAC) CE Phosphoramidite; 5'-DMT-2'-O-methyl-N-PAC-cytidine-3'-CE phosphoramidite; 2'-O-Methyl-C(PAC) Phosphoramidite (PAC = 4-tert-Butylphenoxyacetyl)
EINECS	Contact for details

Quality Control

Our 5'-o-(4,4-Dimethoxytrityl)-n-[[4-(tert-Butyl)Phenoxy]Acetyl]-2'-o-Methylcytidine-3'-(2-Cyanoethyl-N,n-Diisopropyl)Phosphoramidite is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and MS, to ensure it meets the exacting standards required for oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment. We support GMP compliance documentation for advanced therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized (store under inert atmosphere). Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.