Description

5-Fluorouridine-[6-3H] is a tritium-labeled nucleoside analog, a critical tool compound for biochemical and pharmacological research. Its primary value lies in enabling precise metabolic and pharmacokinetic studies through radiotracer techniques. This labeled compound is essential for researchers in pharmaceutical development, oncology research, and molecular biology who require high-specific-activity tracers to investigate cellular uptake, nucleic acid metabolism, and the mechanism of action of fluoropyrimidine-based therapeutics.

Application

• Radiotracer Studies: Used as a labeled precursor in metabolic pathway analysis and nucleic acid synthesis research.
• Pharmacokinetics & Drug Metabolism (DMPK): Tracks the absorption, distribution, metabolism, and excretion (ADME) of 5-fluorouridine and related analogs in vitro and in vivo.
• Oncology Research: Investigates the mechanism of action and cellular incorporation of fluoropyrimidine chemotherapeutic agents.
• Enzyme Assays: Serves as a substrate for studying kinases, nucleoside phosphorylases, and other enzymes involved in nucleoside metabolism.
• Autoradiography & Imaging: Enables precise localization of drug compounds in tissue sections for biodistribution studies.
• Biochemical Pathway Mapping: Traces the incorporation of fluorinated nucleosides into RNA, studying effects on transcription and translation.

Basic Information

Product Name	5-Fluorouridine-[6-3H]
CAS No.	179383-02-9
Molecular Formula	C9H10FN2O6 (³H labeled)
Molecular Weight	Contact for details
Synonyms	5-Fluoro-1-β-D-ribofuranosyluracil-[6-³H]; 5-Fluorouridine (tritium labeled); 5-FUrd-[6-³H]; 1-(β-D-Ribofuranosyl)-5-fluorouracil-[6-³H]; 5-Fluorouridine, [6-³H]-; 5-Fluorouridine (³H); FURD-³H; Tritiated 5-Fluorouridine
EINECS	Contact for details

Quality Control

Every batch of 5-Fluorouridine-[6-3H] is manufactured and analyzed under strict quality systems to ensure high chemical and radiochemical purity, specific activity, and stability. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity (HPLC), specific activity, radiochemical identity, and solvent composition are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as supplied. For long-term storage, keep at -70°C to -80°C. The product is light-sensitive and should be handled in accordance with safe laboratory practices for radioactive materials.

Specification

Item	Specification
Appearance	Clear solution
Identification (HPLC)	Conforms
Chemical Purity (HPLC)	≥ 98%
Radiochemical Purity (HPLC)	≥ 97%
Specific Activity	10-30 Ci/mmol (Contact for exact value)
Solvent	Ethanol:Water solution
Concentration	1 mCi/mL

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.