Description

Guanosine 5'-Monophosphate-[8-3H] Diammonium Salt is a high-specific-activity, tritium-labeled nucleotide essential for advanced biochemical research. This radiolabeled compound serves as a critical tracer for investigating nucleotide metabolism, enzyme kinetics, and nucleic acid synthesis pathways. It is primarily utilized by research scientists and pharmaceutical developers in life sciences, molecular biology, and drug discovery for precise, quantitative analysis.

Application

• Enzyme Assay Substrate: Used as a labeled substrate in studies of kinases, nucleotidyltransferases, and other nucleotide-processing enzymes to measure activity and inhibition.
• Metabolic Pathway Tracing: Enables the tracking and quantification of guanosine monophosphate (GMP) incorporation and turnover in cellular and in vitro systems.
• Nucleic Acid Research: Acts as a precursor for the synthesis of radiolabeled RNA or for studying RNA polymerase activity and nucleotide incorporation rates.
• Receptor Binding Studies: Employed in radioligand binding assays for purinergic receptors that recognize guanine nucleotides.
• Drug Discovery & Development: Facilitates high-throughput screening (HTS) and mechanistic studies for therapeutics targeting nucleotide metabolism, such as antiviral or anticancer agents.
• Biochemical Standard: Serves as a calibrated internal standard in liquid scintillation counting (LSC) and other analytical techniques requiring quantitation of tritium.

Basic Information

Product Name	Guanosine 5'-Monophosphate-[8-3H] Diammonium Salt
CAS No.	179382-90-2
Molecular Formula	C10H18N6O8P * (³H)
Molecular Weight	Approx. 442.2 g/mol (unlabeled form)
Synonyms	8-³H-GMP Diammonium Salt; [8-³H]GMP Diammonium Salt; Guanosine 5'-(dihydrogen phosphate)-[8-³H] Diammonium Salt; Tritiated GMP; 5'-GMP-[8-³H]; 5'-Guanylic Acid-[8-³H] Diammonium Salt; [³H]GMP (Ammonium Salt); Radiolabeled Guanosine Monophosphate
EINECS	Contact for details

Quality Control

Every batch of Guanosine 5'-Monophosphate-[8-3H] Diammonium Salt is manufactured and analyzed under strict quality systems. Product identity, radiochemical purity (>97%), and specific activity are verified using advanced techniques including HPLC with radiometric detection and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity (Ci/mmol or GBq/mmol), solvent composition, and analytical methods is provided with each shipment to ensure traceability and compliance with research standards.

Storage

Preserve in a tightly closed container, protected from light. Store frozen (-20°C or below) in the original solvent as supplied. This product is hygroscopic (moisture-sensitive). For long-term stability, avoid repeated freeze-thaw cycles and store the vial upright to minimize exposure.

Specification

Item	Specification
Appearance	Clear solution
Identification (HPLC)	Conforms to reference standard
Radiochemical Purity (HPLC)	≥ 97%
Specific Activity	10-40 Ci/mmol (Contact for exact value)
Chemical Purity (HPLC, UV)	≥ 95%
Solvent	Ethanol:Water (Specified ratio, contact for details)
Concentration	1 mCi/mL (Contact for exact concentration)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.