Description

n-Blocked-5'-o-Dmt 3'-Ced Iododeoxyuridine Phosphoramidite is a high-purity, modified nucleoside phosphoramidite building block essential for solid-phase oligonucleotide synthesis. This compound matters because it enables the precise incorporation of iododeoxyuridine, a halogenated nucleoside analog, into synthetic DNA strands for advanced research and therapeutic applications. It is primarily needed by biotechnology and pharmaceutical companies engaged in the development of antisense oligonucleotides, aptamers, and diagnostic probes where specific base modifications are required.

Application

• Oligonucleotide Synthesis: Key monomer for the automated solid-phase synthesis of DNA strands containing iododeoxyuridine residues.
• Antisense & siRNA Research: Used to create modified oligonucleotides for gene silencing studies, enhancing nuclease resistance and binding affinity.
• Aptamer Development: Incorporation into SELEX libraries to generate modified aptamers with improved stability and target recognition for diagnostic and therapeutic use.
• Photocrosslinking & Biophysical Probes: Serves as a precursor for creating DNA probes where the iodine atom can be used for heavy-atom labeling in crystallography or for photo-activated crosslinking studies.
• Therapeutic Oligonucleotide Manufacturing: Critical raw material in the GMP-compliant production of oligonucleotide-based drugs.
• Diagnostic Assay Development: Used to synthesize labeled or modified capture probes for sensitive molecular diagnostic platforms.

Basic Information

Product Name	n-Blocked-5'-o-Dmt 3'-Ced Iododeoxyuridine Phosphoramidite
CAS No.	178925-48-9
Molecular Formula	C41H48IN4O8P
Molecular Weight	906.72 g/mol
Synonyms	5-Iodo-2'-deoxyuridine, 5'-O-(4,4'-Dimethoxytrityl)-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5-Iodo-dU Phosphoramidite; 5-Iododeoxyuridine Phosphoramidite; IdU Phosphoramidite; DMT-I-dU-CE Phosphoramidite; 5-I-2'-dU-CE Phosphoramidite; 1-[(2R,4S,5R)-4-([bis(4-methoxyphenyl)(phenyl)methoxy]methyl)-5-([[(2-cyanoethoxy)(diisopropylamino)phosphanyl]oxy]methyl)oxolan-2-yl]-5-iodopyrimidine-2,4-dione
EINECS	Contact for details

Quality Control

Our n-Blocked-5'-o-Dmt 3'-Ced Iododeoxyuridine Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency for critical oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC purity analysis, ³¹P NMR and ¹H NMR for structural confirmation, and moisture content determination. Certificates of Analysis (COA) with full specification data are provided to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dry, freezer. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (³¹P NMR)	Spectrum corresponds to reference
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.