Description

5'-O-(4,4'-Dimethoxytrityl)-2'-Fluoro-D-Cytidine is a high-purity, protected nucleoside phosphoramidite precursor, a critical building block in oligonucleotide synthesis. Its value lies in enabling the precise and efficient incorporation of 2'-fluoro-modified cytidine into synthetic RNA and DNA strands, a key technology for developing advanced therapeutics. This compound is essential for researchers and manufacturers in the pharmaceutical and biotechnology sectors focused on antisense oligonucleotides, siRNA, aptamers, and other nucleic acid-based drugs. The CAS number for this compound is 177086-37-2.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of therapeutic oligonucleotides.
• Antisense Drug Development: For creating oligonucleotides that modulate gene expression by binding to specific RNA sequences.
• siRNA and miRNA Therapeutics: As a critical component in synthesizing small interfering RNA (siRNA) strands, where the 2'-fluoro modification enhances stability and potency.
• Aptamer Production: Used in the synthesis of chemically modified aptamers for diagnostic and therapeutic targeting.
• Research & Development: For academic and industrial R&D into novel nucleic acid chemistries and their biological applications.
• Diagnostic Probe Synthesis: In the manufacture of highly specific, nuclease-resistant probes for molecular diagnostics.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-2'-Fluoro-D-Cytidine
CAS No.	177086-37-2
Molecular Formula	C31H31FN4O7
Molecular Weight	590.60 g/mol
Synonyms	2'-Fluoro-5'-O-(4,4'-dimethoxytrityl)-D-cytidine; 2'-F-D-Cytidine DMT; DMT-2'-F-dC; 5'-O-DMT-2'-fluoro-D-cytidine; 2'-Fluoro-5'-O-DMT-cytidine; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxy-2'-fluoro-D-cytidine (protected form variant); 2'-F-dC(DMT)
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-2'-Fluoro-D-Cytidine is manufactured under strict quality systems suitable for pharmaceutical development. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure consistency and reliability for critical synthesis workflows. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.