Description

5-Formyl-2'-O-Methylcytidine is a chemically modified nucleoside derivative of cytidine, featuring both a formyl group at the 5-position and a methyl group on the 2'-oxygen of the ribose sugar. This specific modification makes it a critical building block and intermediate in advanced oligonucleotide synthesis, particularly for the development of therapeutic nucleic acids. It is essential for researchers and manufacturers in the fields of antisense oligonucleotides, siRNA, and mRNA therapeutics, where precise chemical modifications are required to enhance stability, specificity, and pharmacokinetic properties.

Application

• Intermediate for Modified Oligonucleotide Synthesis: A key raw material in the solid-phase synthesis of oligonucleotides with specific chemical modifications.
• Antisense Drug Development: Used to create oligonucleotides designed to bind to specific RNA sequences to modulate gene expression for therapeutic purposes.
• siRNA and miRNA Research: Incorporated into small interfering RNA (siRNA) or microRNA (miRNA) strands to improve nuclease resistance and cellular uptake.
• mRNA Vaccine and Therapeutic Platforms: Serves as a modified nucleoside triphosphate precursor for in vitro transcription to produce modified mRNA with reduced immunogenicity.
• Structural Biology and Biophysical Studies: Used to probe RNA structure, function, and protein-RNA interactions.
• Diagnostic Probe Development: Incorporated into nucleic acid probes for enhanced detection sensitivity and specificity in diagnostic assays.

Basic Information

Product Name	5-Formyl-2'-O-Methylcytidine
CAS No.	176858-79-0
Molecular Formula	C11H15N3O6
Molecular Weight	285.25 g/mol
Synonyms	2'-O-Methyl-5-formylcytidine; 5-Formyl-2'-O-methylcytidine; 5-Formylcytidine, 2'-O-methyl-; N4-Acetyl-5-formyl-2'-O-methylcytidine (common precursor); 2'-OMe-5-foC; 5-Formyl-2'-methoxycytidine; 1-[(2R,3R,4R,5R)-3,4-Dihydroxy-5-(hydroxymethyl)-3-methyloxolan-2-yl]-5-formylpyrimidine-2,4-dione
EINECS	Contact for details

Quality Control

Every batch of 5-Formyl-2'-O-Methylcytidine is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and consistency. Our quality system is designed to meet the stringent requirements for pharmaceutical intermediates and advanced research chemicals. A comprehensive Certificate of Analysis (COA) detailing purity by HPLC, identity confirmation (NMR, MS), and impurity profiles is provided with each shipment to support your regulatory and R&D documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This compound is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.