Description

5'-O-DMTr-N4-Fmoc-5-Me-dC-Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer essential for solid-phase oligonucleotide synthesis. This compound is specifically designed for the automated synthesis of DNA strands containing 5-methylcytosine, a critical epigenetic modification. It is a vital building block for researchers and manufacturers in the fields of therapeutic oligonucleotides, molecular diagnostics, and genetic research, where precise sequence control and modification are paramount.

Application

• Oligonucleotide Synthesis: Primary building block for the automated solid-phase synthesis of DNA sequences containing 5-methylcytosine (5mC) residues.
• Therapeutic Development: Key raw material in the production of antisense oligonucleotides (ASOs), siRNA, and aptamers where 5mC modification can enhance stability and efficacy.
• Epigenetics Research: Used to synthesize probes and primers for studying DNA methylation patterns and gene expression regulation.
• Diagnostic Reagents: Manufacture of labeled probes for PCR, microarrays, and next-generation sequencing (NGS) applications requiring specific methylation detection.
• CRISPR/Cas9 Guide RNA Synthesis: Component in the synthesis of modified guide RNAs (gRNAs) to improve editing efficiency and specificity.
• Bioconjugation & Labeling: Serves as a starting material for creating functionalized oligonucleotides for sensor development and targeted delivery systems.

Basic Information

Item	Details
Product Name	5'-O-DMTr-N4-Fmoc-5-Me-dC-Phosphoramidite
CAS No.	176755-87-6
Molecular Formula	C52H55N4O8P
Molecular Weight	895.00 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-[(9H-fluoren-9-ylmethoxy)carbonyl]-5-methyl-2'-deoxycytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5-Me-dC Phosphoramidite (Fmoc protected); N4-Fmoc-5-methyl-2'-deoxycytidine-3'-phosphoramidite; 5-Methyl-dC-CE Phosphoramidite (Fmoc); 1-[(2R,3S,5R)-5-[Bis(4-methoxyphenyl)(phenyl)methoxy]-3-[[bis(propan-2-yl)amino-[(2-cyanoethoxy)phosphanyl]oxy]methyl]oxolan-2-yl]-5-methyl-4-[(9H-fluoren-9-ylmethoxy)formamido]pyrimidin-2-one
EINECS	Contact for details

Quality Control

Our 5'-O-DMTr-N4-Fmoc-5-Me-dC-Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, essential for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC, 31P NMR, and 1H NMR, to confirm identity, purity, and suitability for automated synthesizers. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.