Description

5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxy-N4-[(9H-Fluoren-9-Ylmethoxy) Carbonyl]-5-Methylcytidine is a highly specialized, protected nucleoside phosphoramidite precursor, essential for the automated synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of DNA and RNA strands. It is primarily required by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in antisense therapy, PCR primer development, and next-generation sequencing applications.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the solid-phase synthesis of modified DNA strands, particularly those containing 5-methylcytosine bases.
• Antisense & siRNA Therapeutics: For the research and GMP-scale production of therapeutic oligonucleotides targeting genetic diseases and oncology.
• Diagnostic Probe Manufacturing: Used in the synthesis of labeled probes for PCR, qPCR, microarrays, and in-situ hybridization (FISH) assays.
• Next-Generation Sequencing (NGS): Essential for creating custom sequencing primers and adapters with specific chemical modifications.
• Synthetic Biology: Enables the construction of artificial genes and genetic circuits with precise epigenetic markers.
• Academic & Industrial R&D: Serves as a critical building block in biochemistry and molecular biology research for studying gene expression and regulation.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxy-N4-[(9H-Fluoren-9-Ylmethoxy) Carbonyl]-5-Methylcytidine
CAS No.	176755-86-5
Molecular Formula	C46H45N3O9
Molecular Weight	783.88 g/mol
Synonyms	5'-O-DMT-2'-deoxy-N4-Fmoc-5-methylcytidine; 5-Me-dC-CE Phosphoramidite (protected precursor); N4-Fmoc-5-methyl-2'-deoxycytidine, 5'-O-(4,4'-dimethoxytrityl)-; 2'-Deoxy-5-methylcytidine, N4-[(9H-fluoren-9-ylmethoxy)carbonyl]-, 5'-(4,4'-dimethoxytrityl) ether; dCMe Phosphoramidite (Fmoc, DMT); Fmoc-5-methyl-dC(DMT) CE Phosphoramidite; 5-Methyl-2'-deoxycytidine derivative (DMT, Fmoc); Contact for additional synonyms.
EINECS	Contact for details

Quality Control

Every batch of this high-purity building block is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. Quality is verified by HPLC, NMR, and mass spectrometry to ensure identity, purity, and suitability for sensitive oligonucleotide synthesis. Certificates of Analysis (COA) with full chromatographic data are provided for traceability and to support regulatory filings in pharmaceutical and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solvent Residue (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.