Description

5'-O-(4,4'-Dimethoxytrityl)-N4-Isobutyryl-5-Methyl-2'-Deoxycytidine is a critical, high-purity phosphoramidite monomer used in the automated synthesis of oligonucleotides. This compound is essential for ensuring high coupling efficiency and sequence fidelity during solid-phase DNA/RNA synthesis. It is primarily required by biotechnology, pharmaceutical, and diagnostic industries for applications in antisense therapy, PCR primers, probes, and next-generation sequencing.

Application

• Oligonucleotide Synthesis: As a protected monomer for the automated solid-phase synthesis of DNA strands, particularly those containing 5-methylcytosine modifications.
• Therapeutic Oligonucleotide Production: Key raw material in the manufacture of antisense oligonucleotides (ASOs), siRNA, and aptamers for drug development.
• Diagnostic Probe & Primer Manufacturing: Used to synthesize labeled or modified probes and primers for PCR, qPCR, and genomic hybridization assays.
• Research & Development: Essential for academic and industrial R&D in molecular biology, genetics, and synthetic biology for creating custom oligonucleotide libraries.
• Next-Generation Sequencing (NGS): Employed in the synthesis of custom adapters, barcodes, and other oligonucleotide components used in NGS workflows.
• CRISPR Guide RNA Synthesis: Used in the production of chemically modified guide RNAs (gRNAs) to enhance stability and editing efficiency.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-N4-Isobutyryl-5-Methyl-2'-Deoxycytidine
CAS No.	176755-84-3
Molecular Formula	C41H43N3O8
Molecular Weight	705.80 g/mol
Synonyms	5-Methyl-dC(DMT) Phosphoramidite; 5-Me-dC-CE Phosphoramidite; N4-Isobutyryl-5-methyl-2'-deoxycytidine, 5'-O-(4,4'-dimethoxytrityl)-; 5'-O-DMT-N4-ibu-5-methyl-2'-deoxycytidine; 5-Methyl-dC(Ibu) CE Phosphoramidite; DMTr-5-Me-dC(ib) Phosphoramidite precursor; Protected 5-Methyl-2'-deoxycytidine monomer
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-N4-Isobutyryl-5-Methyl-2'-Deoxycytidine is manufactured under strict quality systems to meet the exacting standards of oligonucleotide synthesis. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry. Certificates of Analysis (COA) with detailed chromatographic data are provided to ensure traceability and batch-to-batch consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to maintain stability, due to its hygroscopic and light-sensitive nature. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, DCM, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.