Description

Cytidine, 5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-2'-Deoxy-5-Methyl- is a sophisticated nucleoside derivative, specifically a 5'-DMT-protected 2'-deoxycytidine analog with a methyl substitution at the 5-position of the cytosine base. This compound matters as a critical high-purity building block (phosphoramidite precursor) in the automated synthesis of oligonucleotides, enabling the precise construction of DNA sequences for research and therapeutic applications. It is essential for biotechnology and pharmaceutical companies engaged in antisense therapy development, PCR primer synthesis, and genetic diagnostics, where sequence fidelity and reliable supply are paramount.

Application

• Oligonucleotide Synthesis: Serves as a key protected monomer (phosphoramidite precursor) in solid-phase automated DNA synthesizers.
• Antisense Oligonucleotide (ASO) Production: Used in the manufacture of therapeutic oligonucleotides for targeting specific RNA sequences.
• Research-Grade Primer & Probe Fabrication: Essential for synthesizing custom primers for PCR, qPCR, and sequencing applications in molecular biology labs.
• Diagnostic Kit Development: Incorporated into the production of DNA probes for genetic testing and diagnostic assays.
• Synthetic Biology: A fundamental component for constructing artificial genes and engineered genetic circuits.
• Pharmaceutical R&D: Used in the discovery and development of nucleic acid-based drugs, including aptamers and siRNA components.

Basic Information

Product Name	Cytidine, 5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-2'-Deoxy-5-Methyl-
CAS No.	176755-83-2
Molecular Formula	C37H38N3O7
Molecular Weight	636.72 g/mol
Synonyms	5'-O-DMT-5-Methyl-2'-deoxycytidine; 5-Methyl-2'-deoxycytidine 5'-O-(4,4'-dimethoxytrityl) ether; 5-Me-dC-CE Phosphoramidite precursor; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-5-methyl-2'-deoxycytidine (common protected form); 5-Methyl-dC(DMT); DMT-5-Me-dC; 2'-Deoxy-5-methylcytidine 5'-(4,4'-dimethoxytrityl) derivative
EINECS	Contact for details

Quality Control

This high-value intermediate is produced under strict quality control protocols to ensure consistency and purity essential for oligonucleotide synthesis. Each batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and moisture content, supporting compliance with ISO and cGMP guidelines for pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider desiccant in the storage environment.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.