Description

5'-O-Dmt-N2-Phenylacetylguanosine is a key protected nucleoside derivative used in the synthesis of oligonucleotides. This compound is essential for researchers and manufacturers requiring high-purity building blocks for antisense, siRNA, and diagnostic probe development. It is primarily utilized by pharmaceutical R&D teams, biotechnology companies, and fine chemical suppliers focused on nucleic acid therapeutics and molecular biology applications.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): Serves as a crucial phosphoramidite precursor for the automated synthesis of DNA and RNA strands.
• Antisense Oligonucleotide (ASO) Development: Used in the research and production of therapeutic ASOs targeting genetic diseases.
• siRNA and miRNA Synthesis: A fundamental monomer for constructing short interfering RNA (siRNA) and microRNA (miRNA) sequences for gene silencing studies.
• Diagnostic Probe Manufacture: Employed in creating labeled probes for techniques like FISH (Fluorescence In Situ Hybridization) and PCR.
• Research Scale Nucleic Acid Chemistry: Provides a protected guanosine derivative for exploratory synthesis and method development in academic and industrial labs.
• GMP-Grade Therapeutic Production: Suitable for scaling up under current Good Manufacturing Practice (cGMP) for clinical-grade oligonucleotide APIs.

Basic Information

Product Name	5'-O-Dmt-N2-Phenylacetylguanosine
CAS No.	172965-92-3
Molecular Formula	C39H39N5O8
Molecular Weight	705.76 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N2-phenylacetylguanosine; DMT-Pac-Guanosine; N2-Phenylacetyl-5'-O-(4,4'-dimethoxytrityl)guanosine; Guanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2-(phenylacetyl)amino]-; 2-(Phenylacetamido)-5'-O-(4,4'-dimethoxytrityl)guanosine; Protected Guanosine Phosphoramidite Precursor
EINECS	Contact for details

Quality Control

Every batch of 5'-O-Dmt-N2-Phenylacetylguanosine is manufactured and tested to meet stringent quality standards suitable for pharmaceutical research. Our quality assurance includes comprehensive analytical testing via HPLC, NMR, and mass spectrometry to ensure identity, purity, and consistency. Certificates of Analysis (COA) detailing all specifications are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.