Description

5'-O-(Dimethoxytrityl)-N2-(Dimethylaminomethylidene)-3'-Deoxyguanosine is a key protected nucleoside derivative used as a sophisticated building block in oligonucleotide synthesis. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of DNA and RNA analogs. It is primarily required by pharmaceutical R&D teams, biotechnology companies, and academic research institutions engaged in the development of antisense oligonucleotides, siRNA, and other therapeutic nucleic acids.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A crucial phosphoramidite precursor for the automated synthesis of DNA and RNA sequences.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for antisense, siRNA, and aptamer-based therapies.
• Research-Grade Probe Synthesis: Creation of labeled or modified oligonucleotide probes for diagnostic assays and genomic research.
• Synthesis of Modified Nucleosides: Serves as an advanced intermediate for further chemical modification to create novel nucleoside analogs.
• Process Development & Scale-Up: Used in process chemistry for optimizing and scaling the production of oligonucleotide drugs under cGMP conditions.

Basic Information

Product Name	5'-O-(Dimethoxytrityl)-N2-(Dimethylaminomethylidene)-3'-Deoxyguanosine
CAS No.	172361-60-3
Molecular Formula	C35H38N6O6
Molecular Weight	638.72 g/mol
Synonyms	5'-O-DMT-N2-(Dimethylaminomethylene)-3'-deoxyguanosine; 5'-O-(4,4'-Dimethoxytrityl)-N2-(dimethylaminomethylidene)-2',3'-dideoxyguanosine; DMT-ddG(N2-dmf) Amidite Precursor; N2-(Dimethylaminomethylene)-5'-O-(4,4'-dimethoxytrityl)-2',3'-dideoxyguanosine; 2',3'-Dideoxyguanosine Derivative; Protected 3'-Deoxyguanosine; Guanosine, 2',3'-dideoxy-, 5'-(4,4'-dimethoxytrityl) ether, 2-[(dimethylamino)methylene]amino] deriv.
EINECS	Contact for details

Quality Control

Our 5'-O-(Dimethoxytrityl)-N2-(Dimethylaminomethylidene)-3'-Deoxyguanosine is manufactured under strict quality systems to meet the exacting standards of oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing including HPLC, NMR, and mass spectrometry to ensure high chemical purity and structural integrity. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.