Description

(2-Cyanoethyl) (2,2-Bis(Ethoxycarbonyl)-3-(4,4'-Dimethoxytrityloxy)Propyl-1) N,n-Diisopropylphosphoramidite is a high-purity phosphoramidite monomer, a critical building block in the automated synthesis of oligonucleotides. Its value lies in its role as a key reagent for the controlled, stepwise addition of nucleosides, enabling the production of precise DNA and RNA sequences. This compound is essential for researchers and manufacturers in the fields of therapeutics, diagnostics, and genetic research, where reliable and high-quality synthetic nucleic acids are paramount.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA and RNA strands.
• Therapeutic Oligonucleotide Production: Manufacturing of antisense oligonucleotides, siRNA, and aptamers for pharmaceutical applications.
• Diagnostic Probe Synthesis: Creation of labeled probes for PCR, microarrays, and fluorescence in situ hybridization (FISH).
• Research-Grade Oligo Synthesis: Production of primers, linkers, and gene fragments for academic and industrial R&D.
• Synthesis of Modified Oligonucleotides: Enabling the incorporation of specific functional groups or backbones for advanced applications.
• Scale-Up cGMP Manufacturing: Sourcing of critical raw materials for Good Manufacturing Practice (GMP) production of oligonucleotide-based drugs.

Basic Information

Product Name	(2-Cyanoethyl) (2,2-Bis(Ethoxycarbonyl)-3-(4,4'-Dimethoxytrityloxy)Propyl-1) N,n-Diisopropylphosphoramidite
CAS No.	171285-25-9
Molecular Formula	C43H52N2O9P
Molecular Weight	780.86 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-[(2-cyanoethoxy)(diisopropylamino)phosphino]-3'-O-succinyl-5'-methyluridine 3'-(2-Cyanoethyl) N,N-Diisopropylphosphoramidite; DMT-2'-O-[N,N-Diisopropylamino-(2-cyanoethoxy)phosphinyl]-3'-O-succinyl-5-methyluridine; 2'-O-CE Phosphoramidite of 5'-O-DMT-2'-O-methyluridine-3'-succinate; 2'-O-Methyluridine Phosphoramidite, DMT-protected; RNA Phosphoramidite Monomer; 2'-O-Me-U Phosphoramidite
EINECS	Contact for details

Quality Control

Our (2-Cyanoethyl) (2,2-Bis(Ethoxycarbonyl)-3-(4,4'-Dimethoxytrityloxy)Propyl-1) N,n-Diisopropylphosphoramidite is manufactured under stringent controls to meet the exacting standards of oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC, NMR, and 31P NMR, to ensure high purity and correct structural identity. Certificates of Analysis (COA) with full traceability are provided to support your quality assurance and regulatory needs for research and cGMP applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, light, or ambient humidity will lead to degradation. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.