Description

Alpha,β-Imino Dutp is a chemically modified nucleoside triphosphate analog, specifically a deoxyuridine triphosphate derivative. This compound is of significant value in biochemical research and diagnostic development due to its ability to act as a substrate or inhibitor for various DNA polymerases and related enzymes. It is primarily utilized by researchers in molecular biology, enzymology, and pharmaceutical development for studying enzyme mechanisms, nucleic acid metabolism, and as a potential tool in antiviral or anticancer research.

Application

• Enzyme Kinetics Studies: Used as a substrate analog to investigate the catalytic mechanism and fidelity of DNA polymerases, reverse transcriptases, and other nucleic acid-processing enzymes.
• Antiviral Research: Serves as a potential nucleotide analog for probing the inhibition of viral replication, particularly for viruses encoding their own polymerases.
• Diagnostic Assay Development: Employed in the design and optimization of enzymatic assays, including those for high-throughput screening (HTS) of compound libraries.
• Structural Biology: Aids in X-ray crystallography or cryo-EM studies to determine enzyme-inhibitor complex structures.
• Metabolic Pathway Analysis: Acts as a tracer or probe in studies of nucleotide salvage pathways and DNA repair mechanisms.
• Tool Compound Synthesis: Used as a building block for the synthesis of more complex nucleotide analogs or labeled probes for specific research applications.

Basic Information

Product Name	Alpha,β-Imino Dutp
CAS No.	170428-86-1
Molecular Formula	C9H15N3O13P3
Molecular Weight	505.14 g/mol
Synonyms	2'-Deoxyuridine-5'-triphosphate, α,β-imido analog; α,β-Imino-dUTP; dUTP, α,β-imido; P1-(2'-Deoxyuridin-5'-yl) P3-(dihydroxyphosphinyl) triphosphoroamidate; 2'-Deoxyuridine 5'-(α,β-imidotriphosphate); DUDPNP; α,β-Imido-2'-deoxyuridine-5'-triphosphate; 5'-α,β-Imido-dUTP
EINECS	Contact for details

Quality Control

Our Alpha,β-Imino Dutp is produced under stringent quality management protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant Good Manufacturing Practice (GMP) principles for research chemicals, and specifications can be aligned with customer requirements for specific applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to equilibrate to room temperature before opening to prevent condensation and moisture uptake. For long-term storage, consider desiccants or under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water or buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.