Description

2'-Deoxyuridine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt is a chemically modified nucleotide analog where a non-bridging oxygen atom in the phosphate backbone is replaced by sulfur at the Rp configuration. This modification is critical for its role as a substrate for DNA polymerases and as a tool to confer nuclease resistance in oligonucleotide synthesis. It is an essential reagent for researchers in molecular biology, biochemistry, and medicinal chemistry. The compound is primarily utilized in mechanistic enzymology studies, the synthesis of modified nucleic acids, and as a key component in next-generation sequencing technologies.

Application

• Enzymology & Mechanistic Studies: Used as a substrate analog to investigate the catalytic mechanisms of DNA polymerases, reverse transcriptases, and other nucleic acid-processing enzymes.
• Synthesis of Nuclease-Resistant Oligonucleotides: Incorporated during solid-phase synthesis to produce phosphorothioate-backbone oligonucleotides for antisense, siRNA, and aptamer applications.
• Next-Generation Sequencing (NGS): Serves as a modified nucleotide triphosphate in certain sequencing-by-synthesis platforms to control or modulate polymerase activity.
• Structural Biology: Used in X-ray crystallography and NMR studies to capture snapshots of enzyme-substrate complexes and understand stereospecific interactions.
• Diagnostic Probe Development: Employed in the creation of stabilized nucleic acid probes for in situ hybridization (FISH) and other diagnostic assays requiring enhanced stability.
• Medicinal Chemistry & Drug Discovery: Acts as a building block for developing therapeutic oligonucleotides with improved pharmacokinetic properties.

Basic Information

Product Name	2'-Deoxyuridine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt
CAS No.	170428-85-0
Molecular Formula	C9H14N2O13P3S • xNa+
Molecular Weight	Contact for details
Synonyms	dUTPαS, Rp-isomer, Sodium Salt; 2'-Deoxyuridine-5'-(1-thiotriphosphate), Sp-isomer (stereochemistry inverted); Rp-dUTPαS; Deoxyuridine 5'-(α-thiotriphosphate), Rp diastereomer; 5'-O-[Hydroxy([hydroxy(phosphonooxy)phosphoryl]thio)phosphoryl]-2'-deoxyuridine Sodium Salt; Sp-2'-Deoxyuridine-5'-O-(1-thiotriphosphate) (common vendor listing); α-Thiotriphosphate deoxyuridine, Rp form
EINECS	Contact for details

Quality Control

Our 2'-Deoxyuridine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and diastereomeric excess (D.E.), NMR for structural confirmation, and LC-MS for identity and impurity profiling. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Our quality commitment supports applications requiring high stereochemical purity and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Diastereomeric Excess (D.E.)	≥ 98% Rp-isomer
Water Content (KF)	≤ 3.0%
Biological Activity	Suitable for polymerase incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.