Description

N6-(Diisobutylaminomethylidene)-5'-O-(Dimethoxytrityl)-O2-(Diphenylcarbamoyl)-2'-Deoxyisoguanosine is a sophisticated, high-purity nucleoside phosphoramidite derivative, a critical building block for the solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise incorporation of modified bases, specifically isoguanosine, into synthetic DNA strands for advanced research and therapeutic development. This compound is essential for scientists and manufacturers in the fields of antisense oligonucleotide (ASO) development, therapeutic aptamer discovery, and fundamental nucleic acid chemistry research, where sequence control and base modification are paramount.

Application

• Solid-Phase Oligonucleotide Synthesis: As a phosphoramidite monomer for automated DNA/RNA synthesizers to introduce isoguanosine modifications.
• Therapeutic Aptamer Development: For creating high-affinity nucleic acid ligands (aptamers) with expanded chemical diversity for targeting proteins, cells, or small molecules.
• Antisense & siRNA Research: Incorporation into oligonucleotides designed for gene silencing, where modified bases can enhance stability and binding affinity.
• Expanded Genetic Alphabet Systems: Used in research exploring unnatural base pairs (UBPs) to create synthetic biology systems with novel functionalities.
• Diagnostic Probe Synthesis: Manufacturing of specialized DNA probes for sensitive detection assays, including qPCR and FISH.
• Bioconjugation & Labeling: Serving as a precursor for the site-specific attachment of reporter molecules or functional groups to oligonucleotides.

Basic Information

Product Name	N6-(Diisobutylaminomethylidene)-5'-O-(Dimethoxytrityl)-O2-(Diphenylcarbamoyl)-2'-Deoxyisoguanosine
CAS No.	170023-67-3
Molecular Formula	C52H61N7O7
Molecular Weight	896.10 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N6-(diisobutylaminomethylene)-2'-deoxyisoguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-Deoxyisoguanosine, N6-[(diisobutylamino)methylene]-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-O2-(diphenylcarbamoyl)-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; Iso-dG Phosphoramidite; dIsoG Phosphoramidite; 2'-Deoxyisoguanosine Phosphoramidite (DMT-protected); IsoG-CE Phosphoramidite; CAS 170023-67-3.
EINECS	Contact for details

Quality Control

Every batch of this high-value phosphoramidite is produced and analyzed under strict quality control protocols to ensure consistency and performance in sensitive oligonucleotide synthesis. We guarantee high purity levels suitable for research and GMP-grade manufacturing. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications such as purity (HPLC), identity (NMR, MS), moisture content (KF), and performance in coupling efficiency tests.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Coupling Efficiency Test	≥ 98.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.