Description

1-(5-o-(Dimethoxytrityl)-β-D-2-Deoxyribofuranosyl)-3-Nitropyrrole is a high-purity, protected nucleoside phosphoramidite precursor, a critical building block in automated oligonucleotide synthesis. Its value lies in enabling the precise incorporation of a universal base analog, facilitating the creation of complex DNA/RNA sequences for research and diagnostics. This compound is essential for biotechnology, pharmaceutical R&D, and diagnostic kit manufacturers focused on nucleic acid-based technologies.

Application

• Oligonucleotide Synthesis: As a phosphoramidite monomer for the solid-phase synthesis of DNA and RNA strands.
• Universal Base Incorporation: Used to introduce 3-nitropyrrole, a base with low sequence specificity, into oligonucleotides for applications requiring degenerate binding sites.
• Primer and Probe Development: Key raw material in manufacturing primers, probes, and other oligonucleotides for PCR, sequencing, and hybridization assays.
• Antisense & siRNA Research: Building block for the synthesis of modified oligonucleotides used in therapeutic and functional genomics research.
• Diagnostic Reagent Production: Used in the industrial-scale production of components for molecular diagnostic tests and kits.
• Synthetic Biology: Enables the construction of engineered genetic circuits and artificial genes with specific base-pairing properties.

Basic Information

Product Name	1-(5-o-(Dimethoxytrityl)-β-D-2-Deoxyribofuranosyl)-3-Nitropyrrole
CAS No.	166984-63-0
Molecular Formula	C30H29N3O7
Molecular Weight	543.57 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxy-3-nitropyrrole-1-β-D-ribofuranoside; 1-[5-O-(4,4'-Dimethoxytrityl)-β-D-2-deoxyribofuranosyl]-3-nitropyrrole; 3-Nitropyrrole, 1-[5-O-[bis(4-methoxyphenyl)phenylmethyl]-2-deoxy-β-D-ribofuranosyl]-; DMTr-dP* (Nitropyrrole) Phosphoramidite precursor; Universal Base (3-Nitropyrrole) nucleoside; dP* (Nitropyrrole) nucleoside derivative; 1-(5-O-DMTr-2'-deoxy-β-D-ribofuranosyl)-3-nitropyrrole
EINECS	Contact for details

Quality Control

Our production of this high-value nucleoside derivative adheres to stringent GMP-like guidelines to ensure batch-to-batch consistency and purity critical for oligonucleotide synthesis. Every lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content is provided with each shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Due to its hygroscopic nature, allow the sealed container to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.