Description

2'-O-MOE-5MeU-3'-Phosphoramidite is a high-purity, chemically modified nucleoside phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is critical for introducing specific, nuclease-resistant modifications into synthetic RNA strands, enhancing their stability and therapeutic potential. It is primarily required by biotechnology and pharmaceutical companies engaged in the research and development of antisense oligonucleotides, siRNA therapeutics, and diagnostic probes.

Application

• Synthesis of Antisense Oligonucleotides (ASOs): Key building block for creating oligonucleotides with 2'-O-methoxyethyl (2'-MOE) modifications, which improve target binding affinity and resistance to enzymatic degradation.
• siRNA and RNAi Therapeutic Development: Used to incorporate modified uridine residues into small interfering RNA (siRNA) strands to enhance pharmacokinetic properties and reduce off-target effects.
• Diagnostic Probe Manufacturing: Enables the production of highly stable, labeled oligonucleotide probes for in situ hybridization (ISH) and other molecular diagnostic applications.
• Research Grade Oligonucleotide Synthesis: A vital reagent for academic and industrial research labs synthesizing custom modified RNA sequences for mechanistic studies and drug discovery.
• GMP-Grade Therapeutic Manufacturing: Serves as a critical raw material in the cGMP production of oligonucleotide-based active pharmaceutical ingredients (APIs) for clinical trials and commercial therapeutics.

Basic Information

Product Name	2'-O-MOE-5MeU-3'-Phosphoramidite
CAS No.	163878-63-5
Molecular Formula	C31H41N4O8P
Molecular Weight	628.66 g/mol
Synonyms	5-Methyluridine, 2'-O-(2-methoxyethyl)-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; 2'-O-Methoxyethyl-5-methyluridine-3'-phosphoramidite; 2'-MOE-5-methyl-U Phosphoramidite; 5-Me-2'-MOE-U Phosphoramidite; 1-[3,5-Bis-O-(4-chlorophenyl)-2-deoxy-2-fluoro-β-D-ribofuranosyl]-4-iodopyrazole-3-carboxamide 3'-phosphoramidite (related intermediate); 2'-O-(2-Methoxyethyl)-5-methyluridine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 5-Methyl-2'-O-(2-methoxyethyl)uridine 3'-(2-cyanoethyl diisopropylphosphoramidite)
EINECS	Contact for details

Quality Control

Every batch of 2'-O-MOE-5MeU-3'-Phosphoramidite is manufactured and tested under strict quality systems. We provide comprehensive analytical data to ensure the material meets the stringent purity and consistency requirements for oligonucleotide synthesis. Certificates of Analysis (COA) detailing identity, purity (by HPLC), moisture content, and performance in coupling efficiency tests are available for each lot. Our quality commitment supports compliance with relevant ICH guidelines for pharmaceutical raw materials.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, light, or ambient humidity will lead to decomposition and reduced coupling efficiency. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile
Coupling Efficiency	≥ 99.0% (by DMT assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.