Description

5-Bromouridine 5'-Triphosphate Sodium is a modified nucleotide triphosphate analog where a bromine atom is substituted at the 5-position of the uridine base. This compound is a critical reagent in molecular biology and biochemistry, serving as a substrate for various DNA and RNA polymerases to incorporate brominated nucleotides into nucleic acid chains. It is essential for researchers and manufacturers in the fields of next-generation sequencing (NGS), polymerase chain reaction (PCR), and nucleic acid labeling. The sodium salt form enhances its stability and solubility in aqueous buffers, making it suitable for high-throughput enzymatic applications.

Application

• Next-Generation Sequencing (NGS): Used as a substrate for DNA polymerases during sequencing-by-synthesis to introduce sequence-detectable modifications.
• Polymerase Chain Reaction (PCR): Employed in the synthesis of bromine-labeled DNA probes for applications in hybridization, detection, and microarray analysis.
• Nucleic Acid Labeling & Detection: Facilitates the incorporation of heavy atom labels for structural studies via X-ray crystallography or cryo-electron microscopy.
• Enzymatic RNA Synthesis: Acts as a modified building block for in vitro transcription to produce brominated RNA molecules for structural and functional studies.
• Antiviral & Anticancer Research: Investigated as a potential chain terminator or mutagen in viral and cancer cell replication studies.
• Bioconjugation & Cross-linking: The bromine moiety can serve as a handle for further chemical modification or photo-cross-linking in probe development.

Basic Information

Product Name	5-Bromouridine 5'-Triphosphate Sodium
CAS No.	161848-60-8
Molecular Formula	C9H12BrN2O14P3Na3 (or similar salt form)
Molecular Weight	Contact for details
Synonyms	5-Br-UTP Sodium Salt; BrUTP Na3; 5-Bromo-UTP Trisodium Salt; 5-Bromouridine-5'-triphosphate trisodium; 1-((2R,3S,4R,5R)-5-(4-Bromo-2-oxo-2,3-dihydropyrimidin-1(4H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl) 1-hydroxy triphosphonate trisodium salt; Bromouridine triphosphate sodium; 5-Bromo-UTP
EINECS	Contact for details

Quality Control

Our 5-Bromouridine 5'-Triphosphate Sodium is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. We ensure compliance with relevant industry standards for molecular biology-grade reagents. Certificates of Analysis (COA) with detailed chromatographic data and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥95%
Assay (UV)	≥90% (as sodium salt)
Water Content (KF)	≤5.0%
pH (1% Solution)	6.0 - 8.0
Endotoxin (LAL)	<1.0 EU/mg (for molecular biology grade)
Filter Sterility	0.2 µm filtered (optional)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.