Description

N4-Benzoyl-2'-Deoxy-5'-o-Dmt-Cytidine 3'-Phosphonate Triethylammonium Salt is a key phosphoramidite monomer and a critical building block in the solid-phase synthesis of oligonucleotides. This high-purity intermediate is essential for ensuring high coupling efficiency and sequence fidelity in the production of DNA strands for research, diagnostics, and therapeutic development. It is primarily required by biotechnology and pharmaceutical companies engaged in antisense therapy, PCR primer synthesis, and next-generation sequencing applications.

Application

• Solid-Phase Oligonucleotide Synthesis: As a protected cytidine phosphoramidite monomer for automated DNA synthesizers.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides (ASOs), siRNA, and aptamers for drug development.
• Diagnostic Probe Synthesis: Manufacture of labeled probes and primers for PCR, qPCR, and genomic hybridization assays.
• Research & Development: Synthesis of custom DNA sequences for academic and industrial research in molecular biology.
• Next-Generation Sequencing (NGS): Fabrication of oligonucleotide libraries and adapters for sequencing platforms.
• Synthesis of Modified Oligonucleotides: Building block for incorporating specific modifications into DNA strands.

Basic Information

Product Name	N4-Benzoyl-2'-Deoxy-5'-o-Dmt-Cytidine 3'-Phosphonate Triethylammonium Salt
CAS No.	161664-24-0
Molecular Formula	C46H55N4O9P · C6H15N
Molecular Weight	917.02 g/mol (for the salt form)
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-benzoyl-2'-deoxycytidine 3'-[2-cyanoethyl-N,N-diisopropylphosphoramidite]; dC(Bz) Phosphoramidite; N4-Benzoyl-5'-O-DMT-2'-deoxycytidine-3'-[(2-cyanoethyl)-N,N-diisopropyl]phosphoramidite; 2'-Deoxy-5'-O-(4,4'-dimethoxytrityl)-N4-benzoylcytidine 3'-(2-cyanoethyl diisopropylphosphoramidite); dC-CE Phosphoramidite (Bz protected); Cytidine, 2'-deoxy-N4-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-(2-cyanoethyl diisopropylphosphoramidite)
EINECS	Contact for details

Quality Control

This high-value nucleotide monomer is manufactured under controlled GMP-like conditions to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. Quality is verified through multiple orthogonal analytical techniques including HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and the absence of critical impurities like free amines or moisture. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and solvent content is provided with each batch to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation. For long-term stability, store as recommended and use promptly after opening.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.5%
Assay (NMR)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.