Description

N4-Benzoyl-5-Methylcytidine is a specifically protected nucleoside derivative, a key building block in oligonucleotide synthesis and pharmaceutical research. Its primary value lies in enabling the precise and efficient construction of modified RNA sequences, which is critical for developing advanced therapeutics and molecular biology tools. This high-purity intermediate is essential for researchers and manufacturers in the fields of antisense oligonucleotide (ASO) production, siRNA development, and nucleotide-based drug discovery.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): Used as a phosphoramidite precursor for the automated synthesis of modified RNA strands.
• Antisense & siRNA Therapeutics: A critical raw material in the manufacturing of therapeutic oligonucleotides targeting genetic diseases.
• Research & Diagnostic Probe Development: Enables the creation of labeled or modified probes for genetic analysis and detection.
• Nucleotide Chemistry & Process Development: Serves as a standard or intermediate in scaling up nucleotide manufacturing processes.
• Pharmaceutical Intermediate: Used in the synthesis of more complex nucleoside-based active pharmaceutical ingredients (APIs).

Basic Information

Product Name	N4-Benzoyl-5-Methylcytidine
CAS No.	160107-15-3
Molecular Formula	C17H19N3O6
Molecular Weight	361.35 g/mol
Synonyms	5-Methylcytidine, N4-benzoyl-; 1-[(2R,3R,4R,5R)-3,4-Dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methyl-4-(phenylcarbonylamino)pyrimidin-2-one; N4-Benzoyl-5-methylcytidine; Bz-5-Me-C; N-Benzoyl-5-methylcytidine; 5-Methyl-N4-benzoylcytidine; 5-Methylcytidine N4-benzoate
EINECS	Contact for details

Quality Control

Our N4-Benzoyl-5-Methylcytidine is manufactured under strict quality control protocols to meet the exacting standards of pharmaceutical and biotech applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles as determined by advanced analytical techniques including HPLC, NMR, and mass spectrometry. We adhere to cGMP principles where applicable to ensure batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Related Substances (HPLC)	Total impurities ≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.