Description

5-Methylisocytidine is a modified nucleoside analog of cytidine, where a methyl group is substituted at the 5-position of the cytosine base. This structural modification is critical for its role as a building block in oligonucleotide synthesis, particularly for research into RNA structure, function, and therapeutic applications. It is primarily utilized by pharmaceutical R&D teams, academic researchers, and CROs focused on nucleic acid chemistry, antisense technology, and the development of novel diagnostic probes.

Application

• Oligonucleotide Synthesis: A key phosphoramidite monomer for the solid-phase synthesis of modified RNA and DNA strands.
• Antisense & RNA Therapeutics Research: Used to incorporate stability-enhancing modifications into oligonucleotides for gene silencing and therapeutic applications.
• Structural Biology Studies: Employed to probe RNA structure, folding, and protein-RNA interactions due to its altered base-pairing properties.
• Diagnostic Probe Development: Serves as a component in the manufacture of specialized probes for molecular diagnostics and detection assays.
• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies involving nucleoside-metabolizing enzymes.
• Synthesis of Nucleoside Analogs: Acts as a versatile intermediate for the chemical synthesis of more complex nucleoside derivatives.

Basic Information

Product Name	5-Methylisocytidine
CAS No.	159639-80-2
Molecular Formula	C10H15N3O5
Molecular Weight	257.24 g/mol
Synonyms	5-Methyl-iso-cytidine; 5-Methylisocytidine; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methyl-1,2-dihydropyrimidin-2-one; 5-Methyl-N-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-1,2-dihydropyrimidin-2-imine; 5-Methyl-β-isocytidine; 5-Methyl-isoC
EINECS	Contact for details

Quality Control

Our 5-Methylisocytidine is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry guidelines to support our customers' regulatory and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.