Description

Dmt-2'-F-Dc(Ac) Amidite CAS NO 159414-99-0 is a specialized phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. Its value lies in the site-specific incorporation of a 2'-fluoro-deoxycytidine analog, which significantly enhances the nuclease resistance and binding affinity of the resulting therapeutic oligonucleotides. This high-purity reagent is essential for research and development teams in the pharmaceutical and biotechnology sectors focused on creating advanced antisense, siRNA, and aptamer-based therapeutics.

Application

• Synthesis of therapeutic oligonucleotides including antisense drugs and siRNA for gene silencing applications.
• Manufacture of research-grade DNA/RNA analogs with enhanced stability for biochemical and biophysical studies.
• Production of aptamers used in diagnostic assays and targeted drug delivery systems.
• Development of CRISPR guide RNAs with modified backbones to improve editing efficiency and specificity.
• Key raw material in the production of fluorescence-labeled probes for in situ hybridization and diagnostic kits.

Basic Information

Product Name	Dmt-2'-F-Dc(Ac) Amidite
CAS No.	159414-99-0
Molecular Formula	C41H49FN5O8P
Molecular Weight	797.84 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-fluoro-2'-deoxycytidine, 3'-[(2-Cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite, N4-Acetyl-5'-O-DMT-2'-fluoro-2'-deoxycytidine-3'-CE phosphoramidite, 2'-F-dC(Ac) Phosphoramidite, 2'-Fluoro-deoxycytidine phosphoramidite, DMT-2'F-dC(Ac)-CE Phosphoramidite, 2'-F-dC-CE Phosphoramidite (acetyl protected)
EINECS	Contact for details

Quality Control

Every batch of Dmt-2'-F-Dc(Ac) Amidite is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure it meets the high-purity standards required for oligonucleotide synthesis. Our quality protocols are designed to support GMP-grade production for therapeutic applications. A detailed Certificate of Analysis (COA) providing purity, identity, and moisture content is supplied with each shipment to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dedicated, dry freezer. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.