Description

2'-Fluoro-5'-o-Dimethoxytrityl-N4-Acetyl-D-Cytidine is a high-purity, protected nucleoside derivative essential for oligonucleotide synthesis. This compound matters as a critical building block for the solid-phase synthesis of modified RNA and DNA strands, enabling the creation of stable, therapeutic nucleic acids. It is primarily needed by pharmaceutical R&D teams, CROs/CDMOs specializing in antisense oligonucleotide (ASO) and siRNA drug development, and academic research institutions focused on nucleic acid chemistry.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the solid-phase synthesis of therapeutic oligonucleotides containing 2'-fluoro modifications.
• Antisense Drug Development: Used in the manufacture of antisense oligonucleotides (ASOs) for targeting specific RNA sequences to treat genetic disorders and cancers.
• siRNA Therapeutics: Critical for synthesizing small interfering RNA (siRNA) strands, where the 2'-fluoro modification enhances nuclease resistance and pharmacokinetic properties.
• Aptamer Research: Building block for creating chemically modified aptamers with improved binding affinity and stability for diagnostic and therapeutic applications.
• Chemical Biology Probes: Used to synthesize labeled or modified nucleic acid probes for studying RNA structure, function, and protein interactions.
• Process Development & Scale-up: Serves as a starting material for process chemistry teams at CDMOs scaling up GMP-grade oligonucleotide active pharmaceutical ingredients (APIs).

Basic Information

Product Name	2'-Fluoro-5'-o-Dimethoxytrityl-N4-Acetyl-D-Cytidine
CAS No.	159414-98-9
Molecular Formula	C34H36FN3O8
Molecular Weight	633.67 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-fluoro-N4-acetyl-D-cytidine; D-2'-Fluoro-5'-O-DMT-N4-acetylcytidine; 2'-F-5'-O-DMT-N4-Ac-dC; 2'-Fluoro-5'-O-DMT-N4-acetylcytidine (D-isomer); N4-Acetyl-2'-fluoro-5'-O-(4,4'-dimethoxytrityl)cytidine; 2'-F-DMT-N4-Ac-dC; Protected 2'-Fluoro-D-Cytidine Phosphoramidite Precursor
EINECS	Contact for details

Quality Control

Our 2'-Fluoro-5'-o-Dimethoxytrityl-N4-Acetyl-D-Cytidine is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS). We provide a detailed Certificate of Analysis (COA) with each shipment, documenting critical parameters to support your regulatory filings and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, cool place. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.