Description

5'-O-4,4'-Dimethoxytrityl-5-Fluorouridine is a key nucleoside derivative used as a critical building block in oligonucleotide synthesis. This compound is essential for the solid-phase synthesis of DNA and RNA sequences, particularly those incorporating modified bases for research and therapeutic applications. It is primarily utilized by pharmaceutical R&D teams, biotechnology companies, and academic laboratories focused on antisense oligonucleotides, siRNA, and other nucleic acid-based therapeutics.

Application

• Oligonucleotide Synthesis: Serves as a phosphoramidite precursor for the automated solid-phase synthesis of DNA and RNA strands containing 5-fluorouridine modifications.
• Antisense Drug Development: A vital intermediate in the production of antisense oligonucleotides (ASOs) designed for targeted gene silencing.
• siRNA and miRNA Research: Used to incorporate fluorinated pyrimidines into small interfering RNA (siRNA) and microRNA (miRNA) to study structure-activity relationships and enhance stability.
• Diagnostic Probe Manufacture: Employed in the creation of labeled nucleic acid probes for diagnostic assays and hybridization studies.
• Medicinal Chemistry: Acts as a scaffold for developing novel nucleoside analogs with potential antiviral or anticancer activity.
• Bioconjugation: The dimethoxytrityl (DMT) protecting group allows for selective deprotection and further chemical modifications during complex biomolecule assembly.

Basic Information

Product Name	5'-O-4,4'-Dimethoxytrityl-5-Fluorouridine
CAS No.	157770-09-7
Molecular Formula	C32H31FN2O8
Molecular Weight	590.60 g/mol
Synonyms	5-Fluoro-2'-deoxyuridine 5'-O-(4,4'-dimethoxytrityl) ether; 5'-O-DMT-5-fluorouridine; 1-[(2R,3R,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-(4,4-dimethoxytrityloxymethyl)pyrimidine-2,4-dione; 5-Fluorouridine, 5'-O-(4,4'-dimethoxytrityl)-; DMT-5-Fluorouridine; 5-FUrd(DMT)
EINECS	Contact for details

Quality Control

Our 5'-O-4,4'-Dimethoxytrityl-5-Fluorouridine is produced under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive pharmaceutical research. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry guidelines for advanced pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the sealed container to reach room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Individual impurity ≤ 0.5%
Total Impurities	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.