Description

2'-Dg (Ibu)-2'-Phosphoramidite is a specialized phosphoramidite monomer, a critical building block in the solid-phase synthesis of oligonucleotides. This compound is essential for researchers and manufacturers developing advanced nucleic acid-based therapeutics, diagnostics, and research tools. It is primarily utilized in the pharmaceutical, biotechnology, and life sciences research sectors for the precise construction of modified DNA and RNA sequences.

Application

• Oligonucleotide Synthesis: Key monomer for the automated solid-phase synthesis of DNA and RNA strands, particularly those requiring specific modifications.
• Antisense & siRNA Therapeutics: Production of research-grade and therapeutic oligonucleotides for gene silencing applications.
• Diagnostic Probe Development: Manufacture of labeled probes for PCR, microarrays, and fluorescence in situ hybridization (FISH).
• Aptamer Research: Construction of chemically modified aptamer libraries for binding studies and therapeutic discovery.
• Pharmaceutical R&D: Used in the discovery and development stages of novel nucleic acid drugs.
• Bioconjugation: Serves as an intermediate for attaching oligonucleotides to other molecules, surfaces, or nanoparticles.

Basic Information

Product Name	2'-Dg (Ibu)-2'-Phosphoramidite
CAS No.	157327-99-6
Molecular Formula	C43H55N6O7P
Molecular Weight	798.92 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxyguanosine, N2-isobutyryl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-Deoxyguanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2-N-(2-methyl-1-oxopropyl)-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; dG(Ibu) Phosphoramidite; N-Isobutyryl-2'-deoxyguanosine Phosphoramidite; DMTr-dG(Ibu)-CE Phosphoramidite; Guanosine, 2'-deoxy-, N2-(2-methyl-1-oxopropyl)-, 5'-[bis(4-methoxyphenyl)phenylmethyl] 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; Fast Oligo dG(Ibu) Phosphoramidite
EINECS	Contact for details

Quality Control

Our 2'-Dg (Ibu)-2'-Phosphoramidite is manufactured under strict quality systems suitable for pharmaceutical research and development. Each batch undergoes rigorous analytical testing, including HPLC purity analysis, NMR spectroscopy, and 31P NMR to confirm identity, purity, and reactivity. Certificates of Analysis (COA) with detailed chromatographic data are provided to ensure traceability and batch-to-batch consistency for your critical synthesis workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will degrade product quality and performance.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Moisture Content (KF)	≤ 1.0%
Solubility	Clear, colorless solution in anhydrous acetonitrile
Coupling Efficiency	≥ 99.0% (by trityl assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.