Description

1-(β-D-2-Deoxyribofuranosyl)-3-Nitropyrrole is a specialized nucleoside analog featuring a 3-nitropyrrole base. This compound is of significant value in molecular biology and diagnostic research as a universal base analog, capable of pairing with all four canonical DNA bases with reduced specificity. It is primarily utilized by research institutions, pharmaceutical R&D departments, and biotechnology companies engaged in oligonucleotide synthesis, PCR probe design, and the development of advanced diagnostic assays.

Application

• Oligonucleotide Synthesis: Incorporated as a universal base in primers and probes to reduce sequence bias and enhance binding in complex genomic regions.
• PCR & Molecular Diagnostics: Used in the design of degenerate probes and primers for pathogen detection, SNP analysis, and multiplex assays where sequence variability is high.
• DNA Sequencing & Microarrays: Serves as a neutral placeholder in sequencing primers and array probes to improve hybridization efficiency across diverse target sequences.
• Antisense & siRNA Research: Explored for modifying oligonucleotides to fine-tune binding affinity and specificity in gene silencing applications.
• Forensic Analysis: Potential use in designing probes for challenging forensic samples with degraded or mixed DNA.
• Biochemical Research: A tool for studying DNA-protein interactions and the structural tolerance of polymerases and other enzymes.

Basic Information

Product Name	1-(β-D-2-Deoxyribofuranosyl)-3-Nitropyrrole
CAS No.	157066-48-3
Molecular Formula	C9H11N3O5
Molecular Weight	241.20 g/mol
Synonyms	3-Nitropyrrole 2'-deoxyriboside; 1-(2-Deoxy-β-D-ribofuranosyl)-3-nitropyrrole; 2'-Deoxy-3-nitropyrrole; dPnitro; Universal Base dP; Nitropyrrole deoxyribonucleoside; M1 Nucleoside (related analog); 3-Nitro-1-(2-deoxy-β-D-erythro-pentofuranosyl)-1H-pyrrole
EINECS	Contact for details

Quality Control

Our 1-(β-D-2-Deoxyribofuranosyl)-3-Nitropyrrole is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, water content, and spectroscopic data is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.